ArticleJournal of industrial microbiology & biotechnology2019
Production of medium chain length polyhydroxyalkanoate from acetate by engineered Pseudomonas putida KT2440.
Article in Journal of industrial microbiology & biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 65 citations in OpenAlex.
- Acetate as alternative carbon source for production of mono- and di-rhamnolipids in Pseudomonas putida KT2440.Microbial cell factories · 2026Article
- Multi-layered metabolic remodeling ofMetabolic engineering communications · 2026Article
- Unlocking efficient polyhydroxyalkanoate production by Gram-positive Priestia megaterium using waste-derived feedstocks.Microbial cell factories · 2025Article
- Non-growth substrate ethane perturbs core methanotrophy in obligate methanotrophApplied and environmental microbiology · 2025Article
- Metabolic Engineering ofACS synthetic biology · 2025Article
- UpgradingACS omega · 2025Article
- Metabolic conversion of phenol to polyhydroxyalkanoate (PHA) for addressing dual environmental challenges: A review.Current research in microbial sciences · 2025Review
- Article
- Medium Chain Length Polyhydroxyalkanoate Production by Engineered Pseudomonas gessardii Using Acetate-formate as Carbon Sources.Journal of microbiology (Seoul, Korea) · 2024Article
- Impact of Acetic Acid Supplementation in Polyhydroxyalkanoates Production by Cupriavidus necator Using Mixture-Process Design and Artificial Neural Network.Applied biochemistry and biotechnology · 2024Article
- Autoxidation Catalysis for Carbon-Carbon Bond Cleavage in Lignin.ACS central science · 2023Article
- Perspectives for Using COBioengineering (Basel, Switzerland) · 2023Review
- Citrate Synthase Overexpression ofACS omega · 2023Article
- Leveraging substrate flexibility and product selectivity of acetogens in two-stage systems for chemical production.Microbial biotechnology · 2023Review
- Tuning a high performing multiplexed-CRISPRiMetabolic engineering communications · 2022Article
- Metabolic engineering using acetate as a promising building block for the production of bio-based chemicals.Engineering microbiology · 2022Review
- Utilization of Polymeric Materials toward Sustainable Biodiesel Industry: A Recent Review.Polymers · 2022Review
- Polyhydroxyalkanoates (PHAs) as Biomaterials in Tissue Engineering: Production, Isolation, Characterization.Materials (Basel, Switzerland) · 2022Review
- Effect of short- and medium-chain fatty acid mixture on polyhydroxyalkanoate production byFrontiers in bioengineering and biotechnology · 2022Article
- Advances and trends in microbial production of polyhydroxyalkanoates and their building blocks.Frontiers in bioengineering and biotechnology · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Pseudomonas putida was metabolically engineered to produce medium chain length polyhydroxyalkanoate (mcl-PHA) from acetate, a promising carbon source to achieve cost-effective microbial processes. As acetate is known to be harmful to cell growth, P. putida KT2440 was screened from three Pseudomonas strains (P. putida KT2440, P. putida NBRC14164, and P. aeruginosa PH1) as the host with the highest tolerance to 10 g/L of acetate in the medium. Subsequently, P. putida KT2440 was engineered by amplifying the acetate assimilation pathway, including overexpression of the acs (encoding acetyl-CoA synthetase) route and construction of the ackA-pta (encoding acetate kinase-phosphotransacetylase) pathway. The acs overexpressing P. putida KT2440 showed a remarkable increase of mcl-PHA titer (+ 92%), mcl-PHA yield (+ 50%), and cellular mcl-PHA content (+ 43%) compared with the wild-type P. putida KT2440, which indicated that acetate could be a potential substrate for biochemical production of mcl-PHA by engineered P. putida.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.