ArticleMarine biotechnology (New York, N.Y.)2019
Seminal Plasma Exosomes: Promising Biomarkers for Identification of Male and Pseudo-Males in Cynoglossus semilaevis.
Article in Marine biotechnology (New York, N.Y.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 40 citations in OpenAlex.
- Assessment of two minimally invasive methodologies for sex identification in the European eel, Anguilla anguilla.Journal of fish biology · 2026Article
- Environmental influences on seminal plasma: Molecular and functional insights.The Journal of reproduction and development · 2026Review
- Unveiling the potential of extracellular and outer membrane vesicles in fish aquaculture: a comprehensive review.Frontiers in immunology · 2026Review
- Comparative and Functional Analysis of Exosomal microRNAs During Semelparous Reproduction in Ayu Fish (Journal of extracellular biology · 2025Article
- Functional Study of Four Histone Genes Involved in the Spermatogenesis ofAnimals : an open access journal from MDPI · 2025Article
- Identification and Functional Analysis ofAnimals : an open access journal from MDPI · 2024Article
- MicroRNA Regulation in Infectious Diseases and Its Potential as a Biosensor in Future Aquaculture Industry: A Review.Molecules (Basel, Switzerland) · 2023Review
- Sex-Inclined Piwi-Interacting RNAs in Serum Exosomes for Sex Determination in the Greater Amberjack (International journal of molecular sciences · 2023Article
- Developmental RNA-Seq transcriptomics of haploid germ cells and spermatozoa uncovers novel pathways associated with teleost spermiogenesis.Scientific reports · 2022Article
- Exosome-derived small non-coding RNAs reveal immune response upon grafting transplantation inOpen biology · 2022Article
- Evidence for a Novel Antiviral Mechanism of Teleost Fish: Serum-Derived Exosomes Inhibit Virus Replication through Incorporating Mx1 Protein.International journal of molecular sciences · 2021Article
- miR-23a-3p is involved in drug resistance by directly targeting the influx drug transporter organic anion-transporting polypeptide 2.Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery · 2021Article
- A simple and rapid method for fish sex identification based on recombinase-aided amplification and its use in Cynoglossus semilaevis.Scientific reports · 2021Article
- Identification and characterization of the melanocortin 1 receptor gene (MC1R) in hypermelanistic Chinese tongue sole (Cynoglossus semilaevis).Fish physiology and biochemistry · 2020Article
- Identification and application of piwi-interacting RNAs from seminal plasma exosomes in Cynoglossus semilaevis.BMC genomics · 2020Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
In mammals, small RNAs enclosed in exosomes have been identified as appropriate signatures for disease diagnosis. However, there is limited information on exosomes derived from seminal plasma, and few studies have reported analyzed the composition of exosomes and enclosed small RNAs in fish. The half-smooth tongue sole (Cynoglossus semilaevis) is an economically important fish for aquaculture, and it exhibits sexual dimorphism: the female gender show higher growth rates and larger body sizes than males. Standard karyotype analysis and artificial gynogenesis tests have revealed that this species uses heterogametic sex determination (ZW/ZZ), and so-called sex-reversed pseudo-males exist. In this study, we successfully identified exosomes in the seminal plasma of C. semilaevis; to the best of our knowledge, this is the first report of exosomes in fish seminal plasma. Analysis of the nucleotide composition showed that miRNAs were dominant in the exosomes, and the miRNAs were sequenced and compared to identify signature miRNAs as sexual biomarkers. Moreover, target genes of the signature miRNAs were predicted by sequence matching and annotation. Finally, four miRNAs (dre-miR-141-3P, dre-miR-10d-5p, ssa-miR-27b-3p, and ssa-miR-23a-3p) with significant differential expression in the males and pseudo-males were selected from the signature candidate miRNAs as markers for sex identification, and their expression profiles were verified using real-time quantitative PCR. Our findings could provide an effective detection method for sex differentiation in fish.
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