Evidence map›Paper›PMID 30863691›Full record

Article3 Biotech2019

A hydrolase with esterase activity expressed from a fosmid gene bank prepared from DNA of a North West Himalayan glacier frozen soil sample.

Verruchi Gupta, Inderpal Singh, Paramdeep Kumar, Shafaq Rasool, Vijeshwar Verma

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in 3 Biotech, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.3field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 4 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Verruchi Gupta1School of Biotechnology, Shri Mata Vaishno Devi University, Kakryal, Katra, Jammu and Kashmir 182320 India.
Inderpal Singh1School of Biotechnology, Shri Mata Vaishno Devi University, Kakryal, Katra, Jammu and Kashmir 182320 India.
Paramdeep Kumar3Department of Biotechnology, Guru Nanak Dev University, Amritsar, Punjab 143005 India.
Shafaq Rasool1School of Biotechnology, Shri Mata Vaishno Devi University, Kakryal, Katra, Jammu and Kashmir 182320 India.
Vijeshwar Verma1School of Biotechnology, Shri Mata Vaishno Devi University, Kakryal, Katra, Jammu and Kashmir 182320 India.
Shri Mata Vaishno Devi University · INGuru Nanak Dev University · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Screening of 20,000 clones of a fosmid gene bank, constructed from DNA extracted from North West Himalaya (NWH) glacier soil sample, using functional approach identified 10 esterase/lipase-producing clones. Of these, a clone designated pFG43 with an insert size of 45 kb which produced the highest concentration of enzyme (467.43 U/mg) was sequenced. Clone pFG43 contained 61 open reading frames (ORF) and of these an ORF of 1155 bp designated ME-003, was found to be closely related to a hydrolase from

Indexed as

EsteraseFosmid gene bankFrozen glacier soilMetagenomicsNext-generation sequencing

Identifiers

PMID30863691
PMCPMC6391513
OpenAlexW2915131465

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.