ArticleThe Journal of cell biology1986
A microtubule-associated protein in Drosophila melanogaster: identification, characterization, and isolation of coding sequences.
Article in The Journal of cell biology, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 86 citations in OpenAlex.
- Identification of a novel Polo-like kinase 1 inhibitor that specifically blocks the functions of Polo-Box domain.Oncotarget · 2017Article
- Understanding the Polo Kinase machine.Oncogene · 2015Review
- Sequestration of Polo kinase to microtubules by phosphopriming-independent binding to Map205 is relieved by phosphorylation at a CDK site in mitosis.Genes & development · 2008Article
- Histone deacetylase 6 inhibition compensates for the transport deficit in Huntington's disease by increasing tubulin acetylation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- Characterization of a differentially expressed protein that shows an unusual localization to intracellular membranes in Leishmania major.The Biochemical journal · 2001Article
- Skeletor, a novel chromosomal protein that redistributes during mitosis provides evidence for the formation of a spindle matrix.The Journal of cell biology · 2000Article
- Poly(A)-binding protein I of Leishmania: functional analysis and localisation in trypanosomatid parasites.Nucleic acids research · 2000Article
- Genetic analysis of viable Hsp90 alleles reveals a critical role in Drosophila spermatogenesis.Genetics · 1999Article
- Human autoantibodies reveal titin as a chromosomal protein.The Journal of cell biology · 1998Article
- The Drosophila gene abnormal spindle encodes a novel microtubule-associated protein that associates with the polar regions of the mitotic spindle.The Journal of cell biology · 1997Article
- MHP1, an essential gene in Saccharomyces cerevisiae required for microtubule function.The Journal of cell biology · 1996Article
- The conserved mitotic kinase polo is regulated by phosphorylation and has preferred microtubule-associated substrates in Drosophila embryo extracts.The EMBO journal · 1996Article
- Tubulin domains for the interaction of microtubule associated protein DMAP-85 from Drosophila melanogaster.Molecular and cellular biochemistry · 1996Article
- Stable expression of heterologous microtubule-associated proteins (MAPs) in Chinese hamster ovary cells: evidence for differing roles of MAPs in microtubule organization.The Journal of cell biology · 1994Article
- Identification of ubiquitous high-molecular-mass, heat-stable microtubule-associated proteins (MAPs) that are related to the Drosophila 205-kDa MAP but are not related to the mammalian MAP-4.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Genetic analysis of a Drosophila microtubule-associated protein.The Journal of cell biology · 1992Article
- Analysis of the primary sequence and microtubule-binding region of the Drosophila 205K MAP.The Journal of cell biology · 1990Article
- Identification of a novel nucleotide-sensitive microtubule-binding protein in HeLa cells.The Journal of cell biology · 1990Article
- The conditional inhibition of gene expression in cultured Drosophila cells by antisense RNA.Nucleic acids research · 1989Article
- Identification of microtubule-associated proteins in the centrosome, spindle, and kinetochore of the early Drosophila embryo.The Journal of cell biology · 1989Article
Corrections and comments
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3 authors.
Funding
Abstract
Microtubules and microtubule-associated proteins (MAPs) have been isolated from cultured cells of Drosophila melanogaster by a taxol-dependent polymerization procedure. The principal MAPs are a group of four polypeptides with similar electrophoretic mobilities corresponding to approximately Mr 205,000 (the 205K MAP). These proteins are resistant to precipitation by boiling. One mouse monoclonal antibody and one polyclonal rabbit antiserum specific for the Mr 205,000 MAP were produced and characterized by immunoblotting and indirect immunofluorescence. Both antibody preparations stain the Mr 205,000 molecules and an Mr 255,000 molecule in immunoblots of Drosophila cell homogenates; the rabbit antiserum also stains an Mr 150,000 triplet. Both preparations stain the microtubules of the mitotic spindle, and the rabbit antiserum stains the cytoplasmic microtubules as well. Experiments using affinity-purified rabbit antiserum demonstrate that it is the Mr 205,000 species that is located in the mitotic apparatus and on cytoplasmic microtubules. A random shear genomic library was produced in the expressing vector lambda gt11 and screened with the rabbit antiserum to isolate the DNA sequences encoding these polypeptides. Several cross-hybridizing clones were recovered, shown to encode antigenic determinants in the Mr 205,000 MAP, and characterized by hybridization to Northern blots of mRNA and Southern blots of genomic DNA. Analysis by in situ hybridization reveals that the gene encoding the 205K MAP is located in polytene region 100EF.
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