ArticleScientific reports2019
Contrasting in vitro and in vivo methanol oxidation activities of lanthanide-dependent alcohol dehydrogenases XoxF1 and ExaF from Methylobacterium extorquens AM1.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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39 citing papers in PubMed, 78 citations in OpenAlex.
- Source-Independent Enrichment of Light Lanthanides: Microbial Mobilization, Selective Uptake, and Intracellular Storage.Environmental microbiology · 2026Article
- A frameshift mutation drives divergent biosynthesis of metallophores inbioRxiv : the preprint server for biology · 2026Article
- Investigational treatments of β-cell failure and replacement.Diabetology international · 2026Review
- Functional organization and regulatory logic of theApplied and environmental microbiology · 2026Review
- Identification of overoxidizing and non-overoxidizing NAD-dependent methanol dehydrogenases and implications for synthetic methylotrophy.Nature communications · 2025Article
- Expanding on the ability of trivalent actinides to support microbial alcohol metabolism in evolved methylotrophic bacterium.Communications chemistry · 2025Article
- Aromatic acid metabolism inApplied and environmental microbiology · 2025Article
- Lanthanide-dependent isolation of phyllosphere methylotrophs selects for a phylogenetically conserved but metabolically diverse community.bioRxiv : the preprint server for biology · 2025Article
- Methylobacterium extorquens PA1 utilizes multiple strategies to maintain formaldehyde homeostasis during methylotrophic growth.PLoS genetics · 2025Article
- Emerging role of rare earth elements in biomolecular functions.The ISME journal · 2025Review
- Extensive and diverse lanthanide-dependent metabolism in the ocean.The ISME journal · 2025Article
- A novel engineered strain of Methylorubrum extorquens for methylotrophic production of glycolic acid.Microbial cell factories · 2024Article
- Article
- Structure-driven development of a biomimetic rare earth artificial metalloprotein.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Identification and characterization of a small-molecule metallophore involved in lanthanide metabolism.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Glycine betaine metabolism is enabled inApplied and environmental microbiology · 2024Article
- Different lanthanide elements induce strong gene expression changes in a lanthanide-accumulating methylotroph.Microbiology spectrum · 2023Article
- Methanol-based biomanufacturing of fuels and chemicals using native and synthetic methylotrophs.Synthetic and systems biotechnology · 2023Article
- Core and auxiliary functions of one-carbon metabolism inmSystems · 2023Article
- Compartment-related aspects of XoxF protein functionality in Methylorubrum extorquens DM4 analysed using its cytoplasmic targeting.Antonie van Leeuwenhoek · 2023Article
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4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Lanthanide (Ln) elements are utilized as cofactors for catalysis by XoxF-type methanol dehydrogenases (MDHs). A primary assumption is that XoxF enzymes produce formate from methanol oxidation, which could impact organisms that require formaldehyde for assimilation. We report genetic and phenotypic evidence showing that XoxF1 (MexAM1_1740) from Methylobacterium extorquens AM1 produces formaldehyde, and not formate, during growth with methanol. Enzyme purified with lanthanum or neodymium oxidizes formaldehyde. However, formaldehyde oxidation via 2,6-dichlorophenol-indophenol (DCPIP) reduction is not detected in cell-free extracts from wild-type strain methanol- and lanthanum-grown cultures. Formaldehyde activating enzyme (Fae) is required for Ln methylotrophic growth, demonstrating that XoxF1-mediated production of formaldehyde is essential. Addition of exogenous lanthanum increases growth rate with methanol by 9-12% but does not correlate with changes to methanol consumption or formaldehyde accumulation. Transcriptomics analysis of lanthanum methanol growth shows upregulation of xox1 and downregulation of mxa genes, consistent with the Ln-switch, no differential expression of formaldehyde conversion genes, downregulation of pyrroloquinoline quinone (PQQ) biosynthesis genes, and upregulation of fdh4 formate dehydrogenase (FDH) genes. Additionally, the Ln-dependent ethanol dehydrogenase ExaF reduces methanol sensitivity in the fae mutant strain when lanthanides are present, providing evidence for the capacity of an auxiliary role for ExaF during Ln-dependent methylotrophy.
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