ArticleScientific reports2019
Proteotyping as alternate typing method to differentiate Campylobacter coli clades.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 19 citations in OpenAlex.
- Characterization of aquatic clade 2 and 3 Campylobacter coli isolates from Slovenia reveals admixture with other Campylobacter species.BMC microbiology · 2025Article
- Differentiation ofMicroorganisms · 2022Article
- Transmission pathways ofFrontiers in microbiology · 2022Article
- Article
- Investigation of MALDI-TOF Mass Spectrometry for Assessing the Molecular Diversity ofDiagnostics (Basel, Switzerland) · 2021Article
- Genome and Methylome analysis of a phylogenetic novelMicrobial genomics · 2021Article
- Prediction of Streptococcus uberis clinical mastitis treatment success in dairy herds by means of mass spectrometry and machine-learning.Scientific reports · 2021Article
- Combination of MALDI-TOF Mass Spectrometry and Machine Learning for Rapid Antimicrobial Resistance Screening: The Case ofFrontiers in microbiology · 2021Article
- Characterization ofMicroorganisms · 2020Article
- Whole genome sequencing reveals extended natural transformation in Campylobacter impacting diagnostics and the pathogens adaptive potential.Scientific reports · 2020Article
- Rapid and Culture Free Identification ofFrontiers in microbiology · 2020Article
- MALDI-TOF Mass Spectrometry and Specific Biomarkers: Potential New Key for Swift Identification of Antimicrobial Resistance in Foodborne Pathogens.Microorganisms · 2019Review
- Differentiation ofEuropean journal of microbiology & immunology · 2019Article
- Proteotyping ofFrontiers in microbiology · 2019Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Besides Campylobacter jejuni, Campylobacter coli is the most common bacterial cause of gastroenteritis worldwide. C. coli is subdivided into three clades, which are associated with sample source. Clade 1 isolates are associated with acute diarrhea in humans whereas clade 2 and 3 isolates are more commonly obtained from environmental waters. The phylogenetic classification of an isolate is commonly done using laborious multilocus sequence typing (MLST). The aim of this study was to establish a proteotyping scheme using MALDI-TOF MS to offer an alternative to sequence-based methods. A total of 97 clade-representative C. coli isolates were analyzed by MALDI-TOF-based intact cell mass spectrometry (ICMS) and evaluated to establish a C. coli proteotyping scheme. MLST was used as reference method. Different isoforms of the detectable biomarkers, resulting in biomarker mass shifts, were associated with their amino acid sequences and included into the C. coli proteotyping scheme. In total, we identified 16 biomarkers to differentiate C. coli into the three clades and three additional sub-clades of clade 1. In this study, proteotyping has been successfully adapted to C. coli. The established C. coli clades and sub-clades can be discriminated using this method. Especially the clinically relevant clade 1 isolates can be differentiated clearly.
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