ArticleScientific reports2019
A novel RT-QPCR-based assay for the relative quantification of residue specific m6A RNA methylation.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 55 citations in OpenAlex.
- Single-nucleotide RNA mCell reports · 2026Article
- Dynamic modulation of N6-methyladenosine by ionizing radiation in human cells.Biochemistry and biophysics reports · 2026Article
- m6A-Modified Nucleotide Bases Improve Translation of In Vitro-Transcribed Chimeric Antigen Receptor (CAR) mRNA in T Cells.International journal of molecular sciences · 2026Article
- Therapy-induced mRNA, rRNA and tRNA methylation alterations confer tolerance phenotype in tumor cells: mechanism and implications.International journal of biological sciences · 2026Review
- Mitochondria at the intersections of RNA modifications and metabolism reprogramming implications in cell death, tumor microenvironment, and immunotherapy.Journal of hematology & oncology · 2025Review
- RNA mCell investigation · 2025Article
- NBMC medical genomics · 2025Article
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- When animal viruses meet NVeterinary research · 2024Review
- Comprehensive analysis of mComputational and structural biotechnology journal · 2024Article
- Single-cell mNature neuroscience · 2024Article
- Dynamic landscape of m6A modifications and related post-transcriptional events in muscle-invasive bladder cancer.Journal of translational medicine · 2024Article
- Programmable protein expression using a genetically encoded mNature biotechnology · 2024Article
- Challenges to mapping and defining mRNA (New York, N.Y.) · 2024Article
- METTL3 drives telomere targeting of TERRA lncRNA through m6A-dependent R-loop formation: a therapeutic target for ALT-positive neuroblastoma.Nucleic acids research · 2024Article
- An Overview of Current Detection Methods for RNA Methylation.International journal of molecular sciences · 2024Review
- Studying mFrontiers in molecular neuroscience · 2024Article
- In situ visualization of m6A sites in cellular mRNAs.Nucleic acids research · 2023Article
- mNature cell biology · 2023Article
- SINEUP non-coding RNA activity depends on specific N6-methyladenosine nucleotides.Molecular therapy. Nucleic acids · 2023Article
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Authors and funding
7 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
N6-methyladenosine (m6A) is the most common and abundant RNA modification. Recent studies have shown its importance in the regulation of several biological processes, including the immune response, and different approaches have been developed in order to map and quantify m6A marks. However, site specific detection of m6A methylation has been technically challenging, and existing protocols are long and tedious and often involve next-generation sequencing. Here, we describe a simple RT-QPCR based approach for the relative quantification of candidate m6A regions that takes advantage of the diminished capacity of BstI enzyme to retrotranscribe m6A residues. Using this technique, we have been able to confirm the recently described m6A methylation in the 3'UTR of SOCS1 and SOCS3 transcripts. Moreover, using the method presented here, we have also observed alterations in the relative levels of m6A in specific motifs of SOCS genes in celiac disease patients and in pancreatic β-cells exposed to inflammatory stimuli.
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