Evidence map›Paper›PMID 30861576›Full record

ArticleJournal of anatomy2019

Comparison of indirect peroxidase and avidin-biotin-peroxidase complex (ABC) immunohistochemical staining procedures for c-fos in rat brain.

Jae L Butler, Beverly J Barham, Byron A Heidenreich

Open access · bronzeAbstract readComparative Study
In one paragraph

Article in Journal of anatomy, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.2field-weighted citation impact, top 53% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Jae L ButlerMedical Laboratory Science Program, Department of Health Sciences, Illinois State University, Normal, IL, USA.
Beverly J BarhamMedical Laboratory Science Program, Department of Health Sciences, Illinois State University, Normal, IL, USA.
Byron A HeidenreichDepartment of Psychology and School of Biological Sciences, Illinois State University, Normal, IL, USA.ORCID 0000-0002-7114-0903
Illinois State University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

c-Fos is the product of a gene expressed within neurons in the brain that serves as an anatomical marker of cellular activation. Immunohistochemical staining for c-fos allows a characterization of the effects of many different types of experimental manipulations on neuronal activity, making it a powerful technique for understanding brain, drug and behavior relationships. This study compared visualization of an anti-c-fos primary antibody in 40-μm-thick cryostat sections of formaldehyde-fixed rat brainstem using either a peroxidase enzyme-conjugated secondary antibody (indirect peroxidase) or the peroxidase-conjugated avidin-biotin complex (ABC) method. All sections were treated with H

Indexed as

AnimalsAvidinBiotinBrainHorseradish PeroxidaseImmunohistochemistryMaleProto-Oncogene Proteins c-fosRatsRats, Sprague-DawleyStaining and LabelingAvidinavidin-horseradish peroxidase complexBiotinHorseradish PeroxidaseProto-Oncogene Proteins c-fosavidin-biotin-peroxidase complexc-fosimmunohistochemistry

Identifiers

PMID30861576
PMCPMC6539692
OpenAlexW2921394481

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.