ArticleAMB Express2019
Identification of the two-component guaiacol demethylase system from Rhodococcus rhodochrous and expression in Pseudomonas putida EM42 for guaiacol assimilation.
Article in AMB Express, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 42 citations in OpenAlex.
- Hemoglobin expression reduces aerobic bioconversion of guaiacol in Pseudomonas putida despite non-limiting oxygen.AMB Express · 2026Article
- Biochemical parallels between catabolic pathways for lignin-associated aromatic dimers.Applied and environmental microbiology · 2026Review
- MarK, a Novosphingobium aromaticivorans kinase required for catabolism of multiple aromatic monomers.The Journal of biological chemistry · 2025Article
- Article
- Evaluating lignin degradation under limited oxygen conditions by bacterial isolates from forest soil.Scientific reports · 2024Article
- Carbon-wise utilization of lignin-related compounds by synergistically employing anaerobic and aerobic bacteria.Biotechnology for biofuels and bioproducts · 2024Article
- Harnessing redox proteomics to study metabolic regulation and stress response in lignin-fed Rhodococci.Biotechnology for biofuels and bioproducts · 2023Article
- Bio-Based Valorization of Lignin-Derived Phenolic Compounds: A Review.Biomolecules · 2023Review
- The Catabolic System of Acetovanillone and Acetosyringone inApplied and environmental microbiology · 2022Article
- Cytochromes P450 in the biocatalytic valorization of lignin.Current opinion in biotechnology · 2022Review
- Endophytes in Lignin Valorization: A Novel Approach.Frontiers in bioengineering and biotechnology · 2022Review
- Valorization of lignin components into gallate by integrated biological hydroxylation, O-demethylation, and aryl side-chain oxidation.Science advances · 2021Article
- Characterization of alkylguaiacol-degrading cytochromes P450 for the biocatalytic valorization of lignin.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Lignin Biodegradation by a Cytochrome P450 Enzyme: A Computational Study into Syringol Activation by GcoA.Chemistry (Weinheim an der Bergstrasse, Germany) · 2020Article
- Vanillin Production inApplied and environmental microbiology · 2020Article
- Bacterial Valorization of Lignin: Strains, Enzymes, Conversion Pathways, Biosensors, and Perspectives.Frontiers in bioengineering and biotechnology · 2019Review
- Bacterial conversion of depolymerized Kraft lignin.Biotechnology for biofuels · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
A diversity of softwood lignin depolymerization processes yield guaiacol as the main low molecular weight product. This key aromatic compound can be utilized as a carbon source by several microbial species, most of which are Gram positive bacteria. Microbial degradation of guaiacol is known to proceed initially via demethylation to catechol, and this reaction is catalyzed by cytochrome P450 monooxygenases. These enzymes typically require a set of redox partner proteins, whose number and identities were not described until very recently in the case of guaiacol. In this work we identified two proteins involved in guaiacol demethylation by the actinomycete Rhodococcus rhodochrous. Additionally, we constructed four different polycistronic operons carrying combinations of putative redox partners of this guaiacol demethylation system in an inducible expression plasmid that was introduced into the Gram negative host Pseudomonas putida EM42, and the guaiacol consumption dynamics of each resulting strain were analyzed. All the polycistronic operons, expressing a cytochrome P450 together with a putative ferredoxin reductase from R. rhodochrous and putative ferredoxins from R. rhodochrous or Amycolatopsis ATCC 39116 enabled P. putida EM42 to metabolize and grow on guaiacol as the sole carbon source.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.