ArticleFrontiers in microbiology2019
Rapid Sequencing of Multiple RNA Viruses in Their Native Form.
Article in Frontiers in microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
45 citing papers in PubMed.
- Review
- Molecular Diagnostics of Infectious Diseases.Yonago acta medica · 2026Review
- From reads to results: comparing Oxford Nanopore to Illumina sequencing for citrus virus surveillance.BMC genomics · 2026Article
- Metagenomic screening of the virome of symptomatic tomato plants from La Réunion Island uncovers a complex of viruses including a newly identified whitefly-transmitted polerovirus.Archives of virology · 2026Article
- Long-read sequencing-based analyses of the adult Drosophila brain transcriptome in physiological and pathological settings.BMC genomics · 2025Article
- Investigating the origins of the mutational signatures in cancer.Nucleic acids research · 2025Review
- Comprehensive profiling of host- and virus-derived circular RNAs during vesicular stomatitis virus infection.Frontiers in cellular and infection microbiology · 2025Article
- Nanopore sequencing in veterinary medicine: from concepts to clinical applications.Frontiers in cellular and infection microbiology · 2025Review
- Direct nanopore RNA sequencing of umbra-like virus-infected plants reveals long non-coding RNAs, specific cleavage sites, D-RNAs, foldback RNAs, and temporal- and tissue-specific profiles.NAR genomics and bioinformatics · 2024Article
- Genomics for Arbovirus Surveillance: Considerations for Routine Use in Public Health Laboratories.Viruses · 2024Review
- The Applications of Nanopore Sequencing Technology in Animal and Human Virus Research.Viruses · 2024Review
- Direct RNA sequencing coupled with adaptive sampling enriches RNAs of interest in the transcriptome.Nature communications · 2024Article
- Assessing RNA integrity by digital RT-PCR: Influence of extraction, storage, and matrices.Biology methods & protocols · 2024Article
- The genome of a bunyavirus cannot be defined at the level of the viral particle but only at the scale of the viral population.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Advances in the application of molecular diagnostic techniques for the detection of infectious disease pathogens (Review).Molecular medicine reports · 2023Review
- Evaluation of sequencing and PCR-based methods for the quantification of the viral genome formula.Virus research · 2023Article
- Rapid whole genome sequencing methods for RNA viruses.Frontiers in microbiology · 2023Article
- Molecular Characteristics of Subgenomic RNAs and the Cap-Dependent Translational Advantage Relative to Corresponding Genomic RNAs ofInternational journal of molecular sciences · 2022Article
- Advances in nanopore direct RNA sequencing.Nature methods · 2022Article
- Transcriptome dataset of six human pathogen RNA viruses generated by nanopore sequencing.Data in brief · 2022Article
Corrections and comments
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Authors and funding
20 authors.
Funding
Abstract
Long-read nanopore sequencing by a MinION device offers the unique possibility to directly sequence native RNA. We combined an enzymatic poly-A tailing reaction with the native RNA sequencing to (i) sequence complex population of single-stranded (ss)RNA viruses in parallel, (ii) detect genome, subgenomic mRNA/mRNA simultaneously, (iii) detect a complex transcriptomic architecture without the need for assembly, (iv) enable real-time detection. Using this protocol, positive-ssRNA, negative-ssRNA, with/without a poly(A)-tail, segmented/non-segmented genomes were mixed and sequenced in parallel. Mapping of the generated sequences on the reference genomes showed 100% length recovery with up to 97% identity. This work provides a proof of principle and the validity of this strategy, opening up a wide range of applications to study RNA viruses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.