ArticleNature chemical biology2019
A fluorogenic array for temporally unlimited single-molecule tracking.
Article in Nature chemical biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- CRISPR-Cas-based live cell imaging of genome dynamics.Nature reviews. Genetics · 2026Review
- Chromatin dynamics of the Klf4 locus in mouse pluripotent cells.Scientific reports · 2026Article
- Reactive oxygen species-resistant ultrastable super-resolution DNA framework dots.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- A bright and regenerative array tag with fluorogenic ligands for long-term live-cell nanoscopy and single-molecule tracking.Nature communications · 2025Article
- High-Speed Interferometric Scattering Tracking Microscopy of Compartmentalized Lipid Diffusion in Living Cells.Chemphyschem : a European journal of chemical physics and physical chemistry · 2025Article
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- Nanometer-resolution tracking of single cargo reveals dynein motor mechanisms.Nature chemical biology · 2025Article
- Multicolor Long-Term Single-Particle Tracking Using 10 nm Upconverting Nanoparticles.Nano letters · 2024Article
- Compartmentalization of androgen receptors at endogenous genes in living cells.Nucleic acids research · 2023Article
- Leveraging polymer modeling to reconstruct chromatin connectivity from live images.Biophysical journal · 2023Article
- Live-cell imaging of chromatin contacts opens a new window into chromatin dynamics.Epigenetics & chromatin · 2023Review
- Recent Advances in Single-Molecule Tracking and Imaging Techniques.Annual review of analytical chemistry (Palo Alto, Calif.) · 2023Review
- Single-molecule tracking (SMT): a window into live-cell transcription biochemistry.Biochemical Society transactions · 2023Article
- Fast and parallel nanoscale three-dimensional tracking of heterogeneous mammalian chromatin dynamics.Molecular biology of the cell · 2022Article
- Diffusion and distal linkages govern interchromosomal dynamics during meiotic prophase.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- A Strategy for Accessing Nanobody-Based Electrochemical Sensors for Analyte Detection in Complex Media.ECS sensors plus · 2022Article
- Article
- Impact of chromosomal organization on epigenetic drift and domain stability revealed by physics-based simulations.Biophysical journal · 2021Article
- Spatial Organization of Chromatin: Emergence of Chromatin Structure During Development.Annual review of cell and developmental biology · 2021Review
- Tracking single particles for hours via continuous DNA-mediated fluorophore exchange.Nature communications · 2021Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
We describe three optical tags, ArrayG, ArrayD and ArrayG/N, for intracellular tracking of single molecules over milliseconds to hours. ArrayG is a fluorogenic tag composed of a green fluorescent protein-nanobody array and monomeric wild-type green fluorescent protein binders that are initially dim but brighten ~26-fold on binding with the array. By balancing the rates of binder production, photobleaching and stochastic binder exchange, we achieve temporally unlimited tracking of single molecules. High-speed tracking of ArrayG-tagged kinesins and integrins for thousands of frames reveals novel dynamical features. Tracking of single histones at 0.5 Hz for >1 hour with the import competent ArrayG/N tag shows that chromosomal loci behave as Rouse polymers with visco-elastic memory and exhibit a non-Gaussian displacement distribution. ArrayD, based on a dihydrofolate reductase nanobody array and dihydrofolate reductase-fluorophore binder, enables dual-color imaging. The arrays combine brightness, fluorogenicity, fluorescence replenishment and extended fluorophore choice, opening new avenues for tracking single molecules in living cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.