ArticleScientific reports2019
RNAi-based bioinsecticide for Aedes mosquito control.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Biogenic nanomaterials: A green strategy for sustainable vector-borne disease management.Pest management science · 2026Review
- Small RNA genomics of Aedes aegypti mosquitoes discovers infectious viruses that trigger an RNA interference response.Nature communications · 2026Article
- Vector venom: venomics of Aedes albopictus reveals a large enzyme repertoire and novel cecropins with activity against E. coli.npj drug discovery · 2026Article
- RNA interference (RNAi) for insect pest management: understanding mechanisms, strategies, challenges and future prospects.Biologia futura · 2025Review
- Physiological and Putative Organic Cation Transporter Expression Response to Alizarin Dye Exposure inInsects · 2025Article
- RNA interference mediated mortality in Aedes albopictus: a challenging journey toward species-specific vector control.Parasites & vectors · 2025Article
- Review
- Comparative analysis of convergent and divergent T7 RNA polymerase promoters for the synthesis of dsRNA in vivo and in vitro.RNA (New York, N.Y.) · 2025Article
- Syntaxin-1A Silencing by RNAi Disrupts Growth and Reproduction in the Asian Citrus Psyllid,Insects · 2025Article
- Article
- Review
- Gene silencing in the aedine cell lines C6/36 and U4.4 using long double-stranded RNA.Parasites & vectors · 2024Article
- Challenges of Robust RNAi-Mediated Gene Silencing inInternational journal of molecular sciences · 2024Article
- Article
- RNAi-Mediated Silencing ofInsects · 2024Article
- Combination of computational techniques and RNAi reveal targets in Anopheles gambiae for malaria vector control.PloS one · 2024Article
- Review
- Outlook on RNAi-Based Strategies for ControllingPathogens (Basel, Switzerland) · 2023Review
- Engineered Gut Symbiotic Bacterium-Mediated RNAi for Effective Control ofMicrobiology spectrum · 2023Article
- RNAi-mediated CHS-2 silencing affects the synthesis of chitin and the formation of the peritrophic membrane in the midgut of Aedes albopictus larvae.Parasites & vectors · 2023Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Zika virus infection and dengue and chikungunya fevers are emerging viral diseases that have become public health threats. Their aetiologic agents are transmitted by the bite of genus Aedes mosquitoes. Without effective therapies or vaccines, vector control is the main strategy for preventing the spread of these diseases. Increased insecticide resistance calls for biorational actions focused on control of the target vector population. The chitin required for larval survival structures is a good target for biorational control. Chitin synthases A and B (CHS) are enzymes in the chitin synthesis pathway. Double-stranded RNA (dsRNA)-mediated gene silencing (RNAi) achieves specific knockdown of target proteins. Our goal in this work, a new proposed RNAi-based bioinsecticide, was developed as a potential strategy for mosquito population control. DsRNA molecules that target five different regions in the CHSA and B transcript sequences were produced in vitro and in vivo through expression in E. coli HT115 and tested by direct addition to larval breeding water. Mature and immature larvae treated with dsRNA targeting CHS catalytic sites showed significantly decreased viability associated with a reduction in CHS transcript levels. The few larval and adult survivors displayed an altered morphology and chitin content. In association with diflubenzuron, this bioinsecticide exhibited insecticidal adjuvant properties.
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