ArticleMicrobial cell factories2019
Increasing jojoba-like wax ester production in Saccharomyces cerevisiae by enhancing very long-chain, monounsaturated fatty acid synthesis.
Article in Microbial cell factories, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 29 citations in OpenAlex.
- Engineering Rhodotorula toruloides as a platform organism for de novo synthesis of fatty-acid esters.Nature communications · 2026Article
- Structural basis of VLCFA chain-length determination by the KCS6-CER2 complex in plants.Science advances · 2025Article
- Lipid oxidation driven olefinic aldehyde biosynthesis shapes aged aroma in Qingzhuan tea.Food chemistry: X · 2025Article
- Ectopic expression of wax ester synthase under a wood-specific promoter enhances cell wall production and wood hydrophobicity.Biotechnology for biofuels and bioproducts · 2025Article
- Elevated nitrogen fertilization differentially affects jojoba wax phytochemicals, fatty acids and fatty alcohols.Frontiers in plant science · 2024Article
- Marinobacter: A case study in bioelectrochemical chassis evaluation.Microbial biotechnology · 2023Review
- The production of wax esters in transgenic plants: towards a sustainable source of bio-lubricants.Journal of experimental botany · 2022Review
- Lipases of germinating jojoba seeds efficiently hydrolyze triacylglycerols and wax esters and display wax ester-synthesizing activity.BMC plant biology · 2021Article
- The Studies in Constructing Yeast Cell Factories for the Production of Fatty Acid Alkyl Esters.Frontiers in bioengineering and biotechnology · 2021Review
- Transcription Factor-Based Biosensor for Dynamic Control in Yeast for Natural Product Synthesis.Frontiers in bioengineering and biotechnology · 2021Review
- Biosynthesis of Fatty Alcohols in Engineered Microbial Cell Factories: Advances and Limitations.Frontiers in bioengineering and biotechnology · 2020Review
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Authors and funding
6 authors at 2 institutions in 3 countries.
Funding
Abstract
backgroundFatty acids (FAs) with a chain length of more than 18 carbon atoms (> C18) are interesting for the production of specialty compounds derived from these FAs. These compounds include free FAs, like erucic acid (C22:1-Δ13), primary fatty alcohols (FOHs), like docosanol (C22:0-FOH), as well as jojoba-like wax esters (WEs) (C38-WE to C44-WE), which are esters of (very) long-chain FAs and (very) long-chain FOHs. In particular, FAs, FOHs and WEs are used in the production of chemicals, pharmaceuticals and cosmetic products. Jojoba seed oil is highly enriched in diunsaturated WEs with over 70 mol% being composed of C18:1-C24:1 monounsaturated FOH and monounsaturated FA moieties. In this study, we aim for the production of jojoba-like WEs in the yeast Saccharomyces cerevisiae by increasing the amount of very long-chain, monounsaturated FAs and simultaneously expressing enzymes required for WE synthesis.
resultsWe show that the combined expression of a plant-derived fatty acid elongase (FAE/KCS) from Crambe abyssinica (CaKCS) together with the yeast intrinsic fatty acid desaturase (FAD) Ole1p leads to an increase in C20:1 and C22:1 FAs in S. cerevisiae. We also demonstrate that the best enzyme candidate for C24:1 FA production in S. cerevisiae is a FAE derived from Lunaria annua (LaKCS). The combined overexpression of CaKCS and Ole1p together with a fatty acyl reductase (FAR/FAldhR) from Marinobacter aquaeolei VT8 (MaFAldhR) and a wax synthase (WS) from Simmondsia chinensis (SciWS) in a S. cerevisiae strain, overexpressing a range of other enzymes involved in FA synthesis and elongation, leads to a yeast strain capable of producing high amounts of monounsaturated FOHs (up to C22:1-FOH) as well as diunsaturated WEs (up to C46:2-WE).
conclusionsChanging the FA profile of the yeast S. cerevisiae towards very long-chain monounsaturated FAs is possible by combined overexpression of endogenous and heterologous enzymes derived from various sources (e.g. a marine copepod or plants). This strategy was used to produce jojoba-like WEs in S. cerevisiae and can potentially be extended towards other commercially interesting products derived from very long-chain FAs.
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