ArticleMicrobial cell factories2019
SCRaMbLE generates evolved yeasts with increased alkali tolerance.
Article in Microbial cell factories, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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32 citing papers in PubMed, 60 citations in OpenAlex.
- Advances in large DNA fragment assembly for microbial cell factory engineering.Quantitative biology (Beijing, China) · 2026Review
- Synthetic rewriting technologies in mammalian cells.Nature communications · 2026Review
- Inducible chromosomal rearrangement reveals nonlinear polygenic dosage effects in driving aneuploid yeast traits.Nature communications · 2025Article
- Advances in large-scale DNA engineering with the CRISPR system.Experimental & molecular medicine · 2025Review
- Iterative SCRaMbLE for engineering synthetic genome modules and chromosomes.Nature communications · 2025Article
- Spontaneous and environment induced genomic alterations in yeast model.Cell insight · 2025Review
- Synthetic evolution ofmLife · 2025Review
- Construction and iterative redesign of synXVI a 903 kb synthetic Saccharomyces cerevisiae chromosome.Nature communications · 2025Article
- Bioengineering hybrid artificial life.Frontiers in bioinformatics · 2025Review
- The synthetic future of algal genomes.Cell genomics · 2024Review
- Practical Approaches for the YeastInternational journal of molecular sciences · 2023Review
- A self-propagating, barcoded transposon system for the dynamic rewiring of genomic networks.Molecular systems biology · 2023Article
- Investigation of Genome Biology by Synthetic Genome Engineering.Bioengineering (Basel, Switzerland) · 2023Review
- Mobile Translation Systems Generate Genomically EngineeredACS synthetic biology · 2022Article
- Directed yeast genome evolution by controlled introduction of trans-chromosomic structural variations.Science China. Life sciences · 2022Article
- Enhancement and mapping of tolerance to salt stress and 5-fluorocytosine in synthetic yeast strains via SCRaMbLE.Synthetic and systems biotechnology · 2022Article
- Establishment of a Cre-loxP System Based on a LeakyMicroorganisms · 2022Article
- Combining nucleotide variations and structure variations for improving astaxanthin biosynthesis.Microbial cell factories · 2022Article
- Research progress of pathway and genome evolution in microbes.Synthetic and systems biotechnology · 2022Article
- TAQing2.0 for genome reorganization of asexual industrial yeasts by direct protein transfection.Communications biology · 2022Article
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6 authors at 1 institution in 1 country.
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Abstract
backgroundStrains with increased alkali tolerance have a broad application in industrial, especially for bioremediation, biodegradation, biocontrol and production of bio-based chemicals. A novel synthetic chromosome recombination and modification by LoxP-mediated evolution (SCRaMbLE) system has been introduced in the synthetic yeast genome (Sc 2.0), which enables generation of a yeast library with massive structural variations and potentially drives phenotypic evolution. The structural variations including deletion, inversion and duplication have been detected within synthetic yeast chromosomes.
resultsHaploid yeast strains harboring either one (synV) or two (synV and synX) synthetic chromosomes were subjected to SCRaMbLE. Seven of evolved strains with increased alkali tolerance at pH 8.0 were generated through multiple independent SCRaMbLE experiments. Various of structural variations were detected in evolved yeast strains by PCRTag analysis and whole genome sequencing including two complex structural variations. One possessed an inversion of 20,743 base pairs within which YEL060C (PRB1) was deleted simultaneously, while another contained a duplication region of 9091 base pairs in length with a deletion aside. Moreover, a common deletion region with length of 11,448 base pairs was mapped in four of the alkali-tolerant strains. We further validated that the deletion of YER161C (SPT2) within the deleted region could increase alkali tolerance in Saccharomyces cerevisiae.
conclusionsSCRaMbLE system provides a simple and efficient way to generate evolved yeast strains with enhanced alkali tolerance. Deletion of YER161C (SPT2) mapped by SCRaMbLE can improve alkali tolerance in S. cerevisiae. This study enriches our understanding of alkali tolerance in yeast and provides a standard workflow for the application of SCRaMbLE system to generate various phenotypes that may be interesting for industry and extend understanding of phenotype-genotype relationship.
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