ArticleMicrobial cell factories2019
Exploring small-scale chemostats to scale up microbial processes: 3-hydroxypropionic acid production in S. cerevisiae.
Article in Microbial cell factories, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 37 citations in OpenAlex.
- Sustainable Use of Apple Juice By-product for the Propagation of Vishniacozyma victoriae in Batch and Semicontinuous Processes.Current microbiology · 2026Article
- Scaling up biomass production of Vishniacozyma victoriae and semi-commercial testing for post-harvest control of pome fruit disease.Biotechnology letters · 2026Article
- Advances in metabolic engineering and fermentation for 3-hydroxypropionic acid biosynthesis: a comprehensive review.World journal of microbiology & biotechnology · 2025Review
- Metabolic engineering of Komagataella phaffii for enhanced 3-hydroxypropionic acid (3-HP) production from methanol.Journal of biological engineering · 2025Article
- Mini Bubble Columns for Miniaturizing Scale-Down.Engineering in life sciences · 2025Article
- Transferability of bioprocessing modes for recombinant protease production: from fed-batch to continuous cultivation with Bacillus licheniformis.BMC biotechnology · 2025Article
- EngineeringSynthetic and systems biotechnology · 2024Article
- Engineering the synthetic β-alanine pathway in Komagataella phaffii for conversion of methanol into 3-hydroxypropionic acid.Microbial cell factories · 2023Article
- Production of 3-Hydroxypropionic Acid from Renewable Substrates by Metabolically Engineered Microorganisms: A Review.Molecules (Basel, Switzerland) · 2023Review
- MBRA-2: a Modified Chemostat System to Culture Biofilms.Microbiology spectrum · 2023Article
- Nutrient-Limited Operational Strategies for the Microbial Production of Biochemicals.Microorganisms · 2022Review
- Benchmarking recombinant Pichia pastoris for 3-hydroxypropionic acid production from glycerol.Microbial biotechnology · 2021Article
- Adaptation of central metabolite pools to variations in growth rate and cultivation conditions in Saccharomyces cerevisiae.Microbial cell factories · 2021Article
- Repetitive Fed-Batch: A Promising Process Mode for Biomanufacturing WithFrontiers in bioengineering and biotechnology · 2020Article
- The Rocky Road From Fed-Batch to Continuous Processing WithFrontiers in bioengineering and biotechnology · 2019Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 3 countries.
Funding
Abstract
backgroundThe physiological characterization of microorganisms provides valuable information for bioprocess development. Chemostat cultivations are a powerful tool for this purpose, as they allow defined changes to one single parameter at a time, which is most commonly the growth rate. The subsequent establishment of a steady state then permits constant variables enabling the acquisition of reproducible data sets for comparing microbial performance under different conditions. We performed physiological characterizations of a 3-hydroxypropionic acid (3-HP) producing Saccharomyces cerevisiae strain in a miniaturized and parallelized chemostat cultivation system. The physiological conditions under investigation were various growth rates controlled by different nutrient limitations (C, N, P). Based on the cultivation parameters obtained subsequent fed-batch cultivations were designed.
resultsWe report technical advancements of a small-scale chemostat cultivation system and its applicability for reliable strain screening under different physiological conditions, i.e. varying dilution rates and different substrate limitations (C, N, P). Exploring the performance of an engineered 3-HP producing S. cerevisiae strain under carbon-limiting conditions revealed the highest 3-HP yields per substrate and biomass of 16.6 %C-mol and 0.43 g gCDW
conclusionsSmall-scale chemostat cultures are well suited for the physiological characterization of microorganisms, particularly for investigating the effect of changing cultivation parameters on microbial performance. In our study, optimal conditions for 3-HP production comprised (i) a low dilution rate of 0.04 h
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