ArticlePloS one2019
A single-cell platform for reconstituting and characterizing fatty acid elongase component enzymes.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 32 citations in OpenAlex.
- Integrated Metabolomics, Transcriptomics, and Ultrastructural Assessment of the Myco-Heterotrophic Plant,Plant direct · 2026Article
- Molecular basis of very-long-chain fatty acid elongation by the CER6-GL2 enzyme complex in plant wax biosynthesis.Science advances · 2025Article
- Molecular breeding approaches for the improvement of oil content and fatty acid composition in exotic-derived maize germplasm.The New phytologist · 2025Article
- Genome-wide analysis of KCS family in Medicago reveals MsKCS5's crucial role in abiotic stress adaptation.Plant cell reports · 2025Article
- Genome-wide analysis of the KCS gene family in Medicago truncatula and their expression profile under various abiotic stress.Scientific reports · 2025Article
- The Function of TwoPlants (Basel, Switzerland) · 2025Article
- A β-ketoacyl-CoA synthase encoded by DDP1 controls rice anther dehiscence and pollen fertility by maintaining lipid homeostasis in the tapetum.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Cuticle development and the underlying transcriptome-metabolome associations during early seedling establishment.Journal of experimental botany · 2024Article
- A fatty acid elongase complex regulates cell membrane integrity and septin-dependent host infection by the rice blast fungus.Molecular plant pathology · 2024Article
- Dynamic relationships among pathways producing hydrocarbons and fatty acids of maize silk cuticular waxes.Plant physiology · 2024Article
- The impact of the GLOSSY2 and GLOSSY2-LIKE BAHD-proteins in affecting the product profile of the maize fatty acid elongase.Frontiers in plant science · 2024Article
- Comprehensive analysis of KCS gene family in pear reveals the involvement of PbrKCSs in cuticular wax and suberin synthesis and pear fruit skin formation.Plant molecular biology · 2023Article
- Genome-wide identification and expression analysis of theFrontiers in plant science · 2023Article
- Genome-wide identification and expression analysis of 3-ketoacyl-CoA synthase gene family in rice (Frontiers in plant science · 2023Article
- Single-cell genetic models to evaluate orphan gene function: The case of QQS regulating carbon and nitrogen allocation.Frontiers in plant science · 2023Article
- Tackling functional redundancy of Arabidopsis fatty acid elongase complexes.Frontiers in plant science · 2023Article
- Modifying the yeast very long chain fatty acid biosynthetic machinery by the expression of plant 3-ketoacyl CoA synthase isozymes.Scientific reports · 2022Article
- Article
- Genome-Wide Identification and Expression Profiling ofFrontiers in plant science · 2022Article
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fatty acids of more than 18-carbons, generally known as very long chain fatty acids (VLCFAs) are essential for eukaryotic cell viability, and uniquely in terrestrial plants they are the precursors of the cuticular lipids that form the organism's outer barrier to the environment. VLCFAs are synthesized by fatty acid elongase (FAE), which is an integral membrane enzyme system with multiple components. The genetic complexity of the FAE system, and its membrane association has hampered the biochemical characterization of FAE. In this study we computationally identified Zea mays genetic sequences that encode the enzymatic components of FAE and developed a heterologous expression system to evaluate their functionality. The ability of the maize components to genetically complement Saccharomyces cerevisiae lethal mutants confirmed the functionality of ZmKCS4, ZmELO1, ZmKCR1, ZmKCR2, ZmHCD and ZmECR, and the VLCFA profiles of the resulting strains were used to infer the ability of each enzyme component to determine the product profile of FAE. These characterizations indicate that the product profile of the FAE system is an attribute shared among the KCS, ELO, and KCR components of FAE.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.