ArticleNeurophotonics2019
Investigating the feasibility of channelrhodopsin variants for nanoscale optogenetics.
Article in Neurophotonics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Stress disrupts engram ensembles in lateral amygdala to generalize threat memory in mice.Cell · 2025Article
- Synaptic integration of somatosensory and motor cortical inputs onto spiny projection neurons of mice caudoputamen.The European journal of neuroscience · 2024Article
- Comparing behaviours induced by natural memory retrieval and optogenetic reactivation of an engram ensemble in mice.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Article
- Article
- Metadynamics simulations reveal mechanisms of Na+ and Ca2+ transport in two open states of the channelrhodopsin chimera, C1C2.PloS one · 2024Article
- Formation and fate of an engram in the lateral amygdala supporting a rewarding memory in mice.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2023Article
- Opto-extinction of a threat memory in mice.Brain research bulletin · 2022Article
- A kinetic-optimized CoChR variant with enhanced high-frequency spiking fidelity.Biophysical journal · 2022Article
- Biophysical characterization of light-gated ion channels using planar automated patch clamp.Frontiers in molecular neuroscience · 2022Article
- Neural correlates and determinants of approach-avoidance conflict in the prelimbic prefrontal cortex.eLife · 2021Article
- Channelrhodopsin C1C2: Photocycle kinetics and interactions near the central gate.Biophysical journal · 2021Article
- Dendritic Architecture PredictsFrontiers in neural circuits · 2021Article
- Lights, cytoskeleton, action: Optogenetic control of cell dynamics.Current opinion in cell biology · 2020Review
- The role of neuronal excitability, allocation to an engram and memory linking in the behavioral generation of a false memory in mice.Neurobiology of learning and memory · 2020Article
- Enlightening the frontiers of neurogastroenterology through optogenetics.American journal of physiology. Gastrointestinal and liver physiology · 2020Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Optogenetics has revolutionized the study of circuit function in the brain, by allowing activation of specific ensembles of neurons by light. However, this technique has not yet been exploited extensively at the subcellular level. Here, we test the feasibility of a focal stimulation approach using stimulated emission depletion/reversible saturable optical fluorescence transitions-like illumination, whereby switchable light-gated channels are focally activated by a laser beam of one wavelength and deactivated by an overlapping donut-shaped beam of a different wavelength, confining activation to a center focal region. This method requires that activated channelrhodopsins are inactivated by overlapping illumination of a distinct wavelength and that photocurrents are large enough to be detected at the nanoscale. In tests of current optogenetic tools, we found that ChR2 C128A/H134R/T159C and CoChR C108S and C108S/D136A-activated with 405-nm light and inactivated by coillumination with 594-nm light-and C1V1 E122T/C167S-activated by 561-nm light and inactivated by 405-nm light-were most promising in terms of highest photocurrents and efficient inactivation with coillumination. Although further engineering of step-function channelrhodopsin variants with higher photoconductances will be required to employ this approach at the nanoscale, our findings provide a framework to guide future development of this technique.
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