Evidence map›Paper›PMID 30853419›Full record

ArticleCell chemical biology2019

Refactoring the Cryptic Streptophenazine Biosynthetic Gene Cluster Unites Phenazine, Polyketide, and Nonribosomal Peptide Biochemistry.

Katherine D Bauman, Jie Li, Kazuya Murata, Simone M Mantovani, Samira Dahesh, Victor Nizet, Hanna Luhavaya, Bradley S Moore

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell chemical biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed.

  1. Synthetic and systems biotechnology · 2026
    Review
  2. Article
  3. Article
  4. Review
  5. Review
  6. Article
  7. Article
  8. The taxonomy ofFrontiers in microbiology · 2025
    Article
  9. Article
  10. Review
  11. Article
  12. Article
  13. Article
  14. Article
  15. Natural product reports · 2023
    Review
  16. Article
  17. Article
  18. Article
  19. Journal of natural products · 2022
    Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Katherine D BaumanScripps Institution of Oceanography, University of California at San Diego, La Jolla, CA, USA.
Jie LiScripps Institution of Oceanography, University of California at San Diego, La Jolla, CA, USA.
Kazuya MurataScripps Institution of Oceanography, University of California at San Diego, La Jolla, CA, USA.
Simone M MantovaniScripps Institution of Oceanography, University of California at San Diego, La Jolla, CA, USA.
Samira DaheshDepartment of Pediatrics, University of California at San Diego, La Jolla, CA, USA.
Victor NizetDepartment of Pediatrics, University of California at San Diego, La Jolla, CA, USA; Collaborative to Halt Antibiotic Resistant Microbes, University of California at San Diego, La Jolla, CA, USA; Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California at San Diego, La Jolla, CA, USA.
Hanna LuhavayaScripps Institution of Oceanography, University of California at San Diego, La Jolla, CA, USA. Electronic address: hluhavaya@ucsd.edu.
Bradley S MooreScripps Institution of Oceanography, University of California at San Diego, La Jolla, CA, USA; Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California at San Diego, La Jolla, CA, USA. Electronic address: bsmoore@ucsd.edu.

Funding

Natural Product Genome MiningR01GM085770 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI JENSEN, PAUL R, MOORE, BRADLEY S · 2009 to 2024
$5.8M
Targeted discovery of antibiotics from cave bacteriaR01AI117712 · NIAID · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI MOORE, BRADLEY S, POGLIANO, JOSEPH A · 2016 to 2019
$2.8M
Training Program in Marine BiotechnologyT32GM067550 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI GERWICK, WILLIAM HENRY · 2004 to 2019
$2.0M
NIAID NIH HHS R01 AI117712NIGMS NIH HHS R01 GM085770NIGMS NIH HHS T32 GM067550
6 · The paper itself

Abstract

The disconnect between the genomic prediction of secondary metabolite biosynthetic potential and the observed laboratory production profile of microorganisms is well documented. While heterologous expression of biosynthetic gene clusters (BGCs) is often seen as a potential solution to bridge this gap, it is not immune to many challenges including impaired regulation, the inability to recruit essential building blocks, and transcriptional and/or translational silence of the biosynthetic genes. Here we report the discovery, cloning, refactoring, and heterologous expression of a cryptic hybrid phenazine-type BGC (spz) from the marine actinomycete Streptomyces sp. CNB-091. Overexpression of the engineered spz pathway resulted in increased production and chemical diversity of phenazine natural products belonging to the streptophenazine family, including bioactive members containing an unprecedented N-formylglycine attachment. An atypical discrete adenylation enzyme in the spz cluster is required to introduce the formylglycine moiety and represents a phylogenetically distinct class of adenylation proteins.

Indexed as

Bacterial ProteinsChromatography, High Pressure LiquidMass SpectrometryMultigene FamilyPeptide SynthasesPhenazinesPhylogenyPolyketidesStreptomycesBacterial Proteinsnon-ribosomal peptide synthasePeptide SynthasesPhenazinesPolyketidesstreptophenazine Aadenylation enzymeantibioticcryptic biosynthetic gene clusterheterologous expressionmarine Streptomycesnatural productpromoterrefactoringstreptophenazine

Identifiers

PMID30853419
PMCPMC6525064

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.