ArticleScientific reports2019
Solid-phase synthesis of molecularly imprinted polymer nanolabels: Affinity tools for cellular bioimaging of glycans.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 96 citations in OpenAlex.
- Submolecular modulation of PIEZO1 mechanotransduction with wireless tailor-made nanoswitches.Bioactive materials · 2026Article
- Solid-phase synthesis of high affinity interleukin-6 binders made of gelatin-methacryloyl via a molecular imprinting technique.Materials today. Bio · 2026Article
- A Poly(methacrolein-Nanomaterials (Basel, Switzerland) · 2025Article
- Epitope-imprinted biomaterials with tailor-made molecular targeting for biomedical applications.Bioactive materials · 2025Review
- Review
- Boosting Electrochemical Sensing Performances Using Molecularly Imprinted Nanoparticles.Biosensors · 2024Review
- Article
- Synthetic Catalysts for Selective Glycan Cleavage from Glycoproteins and Cells.Journal of the American Chemical Society · 2024Article
- The Amount of Cross-Linker Influences Affinity and Selectivity of NanoMIPs Prepared by Solid-Phase Polymerization Synthesis.Polymers · 2024Article
- Recent Advances in Molecularly Imprinted Polymers and Their Disease-Related Applications.Polymers · 2023Review
- Imprinted Hydrogel Nanoparticles for Protein Biosensing: A Review.ACS sensors · 2023Review
- Molecularly Imprinted Nanoparticle Ensembles for Rapidly IdentifyingSensors (Basel, Switzerland) · 2023Article
- Molecularly imprinted materials for glycan recognition and processing.Journal of materials chemistry. B · 2022Review
- Iodo Silanes as Superior Substrates for the Solid Phase Synthesis of Molecularly Imprinted Polymer Nanoparticles.Polymers · 2022Article
- Review
- The Evolution of Molecular Recognition: From Antibodies to Molecularly Imprinted Polymers (MIPs) as Artificial Counterpart.Journal of functional biomaterials · 2022Review
- BioMIPs: molecularly imprinted silk fibroin nanoparticles to recognize the iron regulating hormone hepcidin.Mikrochimica acta · 2022Article
- Review
- Epitope-imprinted polymers: Design principles of synthetic binding partners for natural biomacromolecules.Science advances · 2021Article
- Molecularly imprinted polymers by epitope imprinting: a journey from molecular interactions to the available bioinformatics resources to scout for epitope templates.Analytical and bioanalytical chemistry · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hyaluronic acid (HA) is a glycosaminoglycan that plays many roles in health and disease and is a key biomarker of certain cancers. Therefore, its detection at an early stage, by histochemical methods, is of importance. However, intracellular HA can be masked by other HA-binding macromolecules, rendering its visualization somehow problematic. We show that fluorescent molecularly imprinted polymer nanogels (MIP-NPs), can localize and detect intracellular HA. MIP-NPs were synthesized by solid-phase synthesis on glass beads (GBs). GBs were functionalized with terminal alkyne groups on which an azide derivative of the template molecule glucuronic acid was immobilized via click chemistry. Immobilization via the anomeric carbon left the template's carboxyl moiety free to enable strong stoichiometric electrostatic interactions with a benzamidine-based functional monomer, to confer selective recognition to the MIP-NPs. Due to the two-point orientation of the template, the resulting MIP-NPs were endowed with improved binding site homogeneity and specificity, reminiscent of monoclonal antibodies. These synthetic antibodies were then applied for probing and staining HA, of which glucuronic acid is a substructure (epitope), on human epidermal cells. Their excellent sensitivity, small size and water compatibility, enabled the MIP-NPs to visualize HA, as evidenced by confocal fluorescence micrographs.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.