Evidence map›Paper›PMID 30850239›Full record

ArticleVaccine2019

ELISA units, IgG subclass ratio and avidity determined functional activity of mouse anti-Pfs230 antibodies judged by a standard membrane-feeding assay with Plasmodium falciparum.

Kazutoyo Miura, Bingbing Deng, Yimin Wu, Luwen Zhou, Thao P Pham, Ababacar Diouf, Chia-Kuei Wu, Shwu-Maan Lee, Jordan L Plieskatt, Merribeth J Morin and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Vaccine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
4.0field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 26 citations in OpenAlex.

  1. Article
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  6. Malaria Genomics, Vaccine Development, and Microbiome.Pathogens (Basel, Switzerland) · 2023
    Review
  7. Article
  8. Article
  9. Article
  10. Article
  11. Frontiers in cellular and infection microbiology · 2022
    Review
  12. Article
  13. Article
  14. Article
  15. Review
  16. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Kazutoyo MiuraLaboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USA. Electronic address: kmiura@niaid.nih.gov.
Bingbing DengLaboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USA.
Yimin WuPATH's Malaria Vaccine Initiative (MVI), Washington, DC 20001, USA.
Luwen ZhouLaboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USA.
Thao P PhamLaboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USA.
Ababacar DioufLaboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USA.
Chia-Kuei WuPATH's Malaria Vaccine Initiative (MVI), Washington, DC 20001, USA.
Shwu-Maan LeePATH's Malaria Vaccine Initiative (MVI), Washington, DC 20001, USA.
Jordan L PlieskattPATH's Malaria Vaccine Initiative (MVI), Washington, DC 20001, USA.
Merribeth J MorinPATH's Malaria Vaccine Initiative (MVI), Washington, DC 20001, USA.
Carole A LongLaboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852, USA.
National Institutes of Health · USU.S. President's Malaria Initiative · US

Funding

Host Immune Responses to Antigens of Malaria ParasitesZIAAI001020 · NIAID · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · PI LONG, CAROLE · 2009 to 2025
$13.6M
Intramural NIH HHS Z99 AI999999
6 · The paper itself

Abstract

The standard membrane-feeding assay (SMFA) is a functional assay that has been used to inform the development of transmission-blocking vaccines (TBV) against Plasmodium falciparum malaria. For Pfs230, a lead target antigen for TBV development, a few studies have tested either a single anti-Pfs230 polyclonal or monoclonal antibody (one antibody per study) at serial dilutions and showed a dose-dependent response. Further, there have been reports that the SMFA activity of anti-Pfs230 polyclonal and monoclonal antibodies were enhanced in the presence of complement. However, no analysis has been performed with multiple samples, and the impact of anti-Pfs230 antibody titers, IgG subclass profile and avidity were evaluated together in relation to transmission-reducing activity (TRA) by SMFA. In this report, a total of 39 unique anti-Pfs230 IgGs from five different mouse immunization studies were assessed for their ELISA units (EU), IgG2/IgG1 ratio and avidity by ELISA, and the functionality (% transmission-reducing activity, %TRA) by SMFA. The mice were immunized with Pfs230 alone, Pfs230 conjugated to CRM

Indexed as

Antibody AffinityEnzyme-Linked Immunosorbent AssayAnimalsAnophelesAntibodies, ProtozoanAntigens, ProtozoanFemaleImmunizationImmunoglobulin GMalaria, FalciparumMalaria VaccinesMicePlasmodium falciparumAntibodies, ProtozoanAntigens, ProtozoanImmunoglobulin GMalaria VaccinesAvidityIgG subclassPfs230Plasmodium falciparumStandard membrane-feeding assayTransmission-blocking vaccine

Identifiers

PMID30850239
PMCPMC6718089
OpenAlexW2920685957

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.