ArticleBMC veterinary research2019
Molecular detection and phylogenetic analysis of Peste des petits ruminants virus circulating in small ruminants in eastern Amhara region, Ethiopia.
Article in BMC veterinary research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 30 citations in OpenAlex.
- Prioritizing smallholder animal health needs in East Africa, West Africa, and South Asia using three approaches: Literature review, expert workshops, and practitioner surveys.Preventive veterinary medicine · 2021Pooled it
- Is PPR Epidemiological Research Addressing Global Eradication Priorities? A Scoping Review (2015-2025).Viruses · 2026Article
- Isolation and molecular characterization of peste des petits ruminants' virus in small ruminants from outbreak cases in Eastern Ethiopia.BMC veterinary research · 2026Article
- Molecular Detection and Seroprevalence Study of Peste des Petits Ruminants Virus in Small Ruminants in Central Ethiopia.Veterinary medicine international · 2026Article
- Global evolutionary dynamics of peste des petits ruminants virus reveals geographic lineage structuring and clade-defining mutational signatures.Frontiers in microbiology · 2026Article
- Diagnostic and vaccination challenges in the PPR pre-eradication era: Identifying gaps and potential unintended consequences.Journal of advanced veterinary and animal research · 2025Article
- Comparative virulence study of locally circulating peste des petits ruminants virus (PPRV) strains in indigenous Ethiopian goats.BMC veterinary research · 2025Article
- Seroprevalence, and molecular detection of Peste des Petits Ruminants in selected districts of Tigray Region, Ethiopia.Virology journal · 2025Article
- Article
- Empirical and model-based evidence for a negligible role of cattle in peste des petits ruminants virus transmission and eradication.Communications biology · 2024Article
- Recapitulation of Peste des Petits Ruminants (PPR) Prevalence in Small Ruminant Populations of Pakistan from 2004 to 2023: A Systematic Review and Meta-Analysis.Veterinary sciences · 2024Review
- Molecular Epidemiology of Peste Des Petits Ruminants Virus in West Africa: Is Lineage IV Replacing Lineage II in Burkina Faso?Viruses · 2024Article
- Seroprevalence and associated risk factors of peste des petits ruminants in sheep and goats in three districts of the Central Oromia Region, Ethiopia.Frontiers in veterinary science · 2024Article
- Complete genome of a 2014 isolate of peste des petits ruminants virus from Ethiopia.Microbiology resource announcements · 2023Article
- A Clinical, Pathological, Epidemiological and Molecular Investigation of Recent Outbreaks of Peste des Petits Ruminants Virus in Domestic and Wild Small Ruminants in the Abu Dhabi Emirate, United Arab Emirates.Veterinary sciences · 2023Article
- Sero-Prevalence and Associated Risk Factors of Peste Des Petits Ruminants in Dera and Gerar Jarso Districts of Oromia Region, Ethiopia.Veterinary medicine (Auckland, N.Z.) · 2023Article
- Molecular Epidemiology and Phylogenetic Analysis of Peste des Petits Ruminants Virus Circulating in Sheep in Bangladesh.Transboundary and emerging diseases · 2023Article
- Isolation and Molecular Characterization ofAdvances in virology · 2022Article
- Wide circulation of peste des petits ruminants virus in sheep and goats across Nigeria.The Onderstepoort journal of veterinary research · 2021Article
- Peste des petits ruminants in Africa: a review of currently available molecular epidemiological data, 2020.Archives of virology · 2020Review
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPeste des Petits Ruminants (PPR) is a severe, highly infectious and fatal viral disease of small ruminants. Four lineages of PPR virus have been identified globally based on sequence analysis of the nucleoprotein (N) and fusion (F) gene. The aim of this study was to isolate and genetically characterize recently circulating PPR virus in small ruminants in the eastern Amhara region in Ethiopia. A total of 28 anti-mortem samples (gum debris, nasal and ocular swab) were collected from clinically suspicious animals and examined for the presence of PPRV by a one-step RT-PCR assay. Samples positive with RT-PCR were subjected to isolation of the virus which were subsequently genetically characterized by sequencing of the nucleoprotein (N) gene and phylogenetic analysis of PPR virus (PPRV) strains.
resultsOf the 28 clinical samples examined, 46.4% were positive with RT-PCR for viral nucleic acid. The PPRV was successfully isolated on CHS-20 cell line with the ovine signaling lymphocyte activation molecule (SLAM) receptor expressed on the cell surface and confirmed with RT-PCR and IFAT assay. The nucleotide sequence and phylogenetic analysis indicated that the PPRV obtained were clustered genetically with Lineage IV isolates of the virus.
conclusionThe successful isolation of the virus and molecular findings of this study confirmed active lineage IV PPRV infections among populations of sheep and goats in eastern Amhara, suggesting risks for potential spread of the disease to currently free areas. Thus, we recommend systematic vaccination to contain outbreaks in affected districts and geographically linked surrounding districts to which the disease could potentially spread due to different epidemiological linkages.
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