ArticlePloS one2019
High-fat diet impacts more changes in beta-cell compared to alpha-cell transcriptome.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 23 citations in OpenAlex.
- Article
- Semaglutide treatment in MOSH is associated with altered DNA methylation patterns of genes related to glycolipid metabolism.Scientific reports · 2026Article
- Transient ER stress cell-autonomously promotes beta cell cycling in mice.Diabetologia · 2026Article
- Dynamic Ca2+-Dependent Transcription Links Metabolic Stress to Impaired β-Cell Identity.Diabetes · 2025Article
- Article
- New Insights and Potential Therapeutic Interventions in Metabolic Diseases.International journal of molecular sciences · 2023Review
- FALCON systematically interrogates free fatty acid biology and identifies a novel mediator of lipotoxicity.Cell metabolism · 2023Article
- A beta cell subset with enhanced insulin secretion and glucose metabolism is reduced in type 2 diabetes.Nature cell biology · 2023Article
- FALCON systematically interrogates free fatty acid biology and identifies a novel mediator of lipotoxicity.bioRxiv : the preprint server for biology · 2023Article
- Pancreatic Islet Cells Response to IFNγ Relies on Their Spatial Location within an Islet.Cells · 2022Article
- The Human and Mouse Islet Peptidome: Effects of Obesity and Type 2 Diabetes, and Assessment of Intraislet Production of Glucagon-like Peptide-1.Journal of proteome research · 2021Article
- Stage-specific transcriptomic changes in pancreatic α-cells after massive β-cell loss.BMC genomics · 2021Article
- Menin-regulated Pbk controls high fat diet-induced compensatory beta cell proliferation.EMBO molecular medicine · 2021Article
- Single-Cell Transcriptional Profiling of Mouse Islets Following Short-Term Obesogenic Dietary Intervention.Metabolites · 2020Article
- Ion Channels of the Islets in Type 2 Diabetes.Journal of molecular biology · 2020Review
- VEGF-B ablation in pancreatic β-cells upregulates insulin expression without affecting glucose homeostasis or islet lipid uptake.Scientific reports · 2020Article
- Advances in Research on Diabetes by Human Nutriomics.International journal of molecular sciences · 2019Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Characterization of endocrine-cell functions and associated molecular signatures in diabetes is crucial to better understand why and by which mechanisms alpha and beta cells cause and perpetuate metabolic abnormalities. The now recognized role of glucagon in diabetes control is a major incentive to have a better understanding of dysfunctional alpha cells. To characterize molecular alterations of alpha cells in diabetes, we analyzed alpha-cell transcriptome from control and diabetic mice using diet-induced obesity model. To this aim, we quantified the expression levels of total mRNAs from sorted alpha and beta cells of low-fat and high-fat diet-treated mice through RNAseq experiments, using a transgenic mouse strain allowing collections of pancreatic alpha- and beta-cells after 16 weeks of diet. We now report that pancreatic alpha cells from obese hyperglycemic mice displayed minor variations of their transcriptome compared to controls. Depending on analyses, we identified 11 to 39 differentially expressed genes including non-alpha cell markers mainly due to minor cell contamination during purification process. From these analyses, we identified three new target genes altered in diabetic alpha cells and potently involved in cellular stress and exocytosis (Upk3a, Adcy1 and Dpp6). By contrast, analysis of the beta-cell transcriptome from control and diabetic mice revealed major alterations of specific genes coding for proteins involved in proliferation and secretion. We conclude that alpha cell transcriptome is less reactive to HFD diet compared to beta cells and display adaptations to cellular stress and exocytosis.
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