ArticleVeterinary medicine and science2019
Effects of egg yolk and soybean lecithin on sperm quality determined by computer-assisted sperm analysis and confocal laser scanning microscope in chilled canine sperm.
Article in Veterinary medicine and science, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 29 citations in OpenAlex.
- TRIS-Egg Yolk Extender Attenuates Seminolipid and Sphingomyelin Loss in Chilled Canine Semen: An LC-HRMS Lipidomic and CASA Study.Animals : an open access journal from MDPI · 2026Article
- Antibiotic-free short-term storage of canine sperm at 5 °C preserves functional and mitochondrial integrity.Frontiers in veterinary science · 2026Article
- Impact of Cryopreservation Extenders on Epigenetic Changes in Bull Sperm: H19 and MEG3 Methylation.Veterinary medicine and science · 2025Article
- Short communication: Pharmacological semen collection in dogs via urethral catheterization: evaluation of cardiorespiratory stability and semen quality under continuous dexmedetomidine infusion.Veterinary research communications · 2025Article
- Effects of Cryoprotectant Combinations on Post-Thawed Sperm Quality, Biomolecular Changes, DNA Methylation, and Pregnancy Rates in Boer Goat Semen.Veterinary sciences · 2025Article
- Dietary Phospholipids and Their Impact on Crustacean Physiology: Growth, Metabolism, Immunity, and Beyond.Aquaculture nutrition · 2025Review
- Comprehensive overview of the toxicities of small-molecule cryoprotectants for carnivorous spermatozoa: foundation for computational cryobiotechnology.Frontiers in toxicology · 2025Review
- Enhancing canine semen quality through a second centrifugation after 48 hours of storage: a comparative study.Acta veterinaria Scandinavica · 2024Article
- The Effect of Different Extenders on the Quality Characteristics of European Red Deer Epididymal Sperm Stored at 5 °C.Animals : an open access journal from MDPI · 2022Article
- Perspectives on lecithin from egg yolk: Extraction, physicochemical properties, modification, and applications.Frontiers in nutrition · 2022Review
- The Use of κ-Carrageenan in Egg Yolk Free Extender Improves the Efficiency of Canine Semen Cryopreservation.Animals : an open access journal from MDPI · 2021Article
- Evaluation of Chilled Dog Semen Extended With Sperm Activator.Frontiers in veterinary science · 2021Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The reduction of spermatozoa survival time is a major problem of canine chilled sperm for artificial insemination. The aim of the study was to improve the quality of canine chilled sperm during storage time. We therefore, evaluated the effects of eight treatments with different levels of soybean lecithin concentration (1, 3 and 5%) and egg yolk (20%) in Tris-citric-fructose or Tris-citric-fructose-mineral salts extender on chilled canine sperm quality during 10 days of storage. The sperm motility was analysed by computer-assisted sperm analysis (CASA), whereas plasma membrane integrity, acrosome membrane integrity and mitochondrial membrane potential parameters were determined using a fluorescent staining combination of propidium iodide (PI), Hoechst 33342 (H342), fluorescein isothiocyanate-conjugated Pisum sativum agglutinin (FITC-PSA) and 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl-carbocyanine iodide (JC-1) by confocal laser scanning microscope. The results showed that egg yolk was found to be better than soybean lecithin in Tris-citric-fructose or Tris-citric-fructose-mineral salts extender for maintaining the quality of chilled canine sperm within 10 days of storage (P < 0.05). Although egg yolk in Tris-citric-fructose extender could maintain the motility better than other extenders, egg yolk in Tris-citric-fructose-mineral salts extender was the highest in intact plasma membrane, intact acrosome membrane and high mitochondrial membrane potential (P < 0.05). In contrast, the sperm quality of soybean lecithin in Tris-citric-fructose-mineral salts extender was lower than that of soybean lecithin in Tris-citric-fructose extender, and soybean lecithin 1% was greater than soybean lecithin 3% and 5% in plasma membrane integrity, acrosome membrane integrity and mitochondrial membrane potential (P < 0.05). In conclusion, soybean lecithin cannot replace egg yolk in Tris-citric-fructose or Tris-citric-fructose-mineral salts extenders, and egg yolk in Tris-citric-fructose-mineral salts extender is superior to other extenders in chilling canine sperm.
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