ArticleGenes, brain, and behavior2019
Housing mice in the individually ventilated or open cages-Does it matter for behavioral phenotype?
Article in Genes, brain, and behavior, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
21 citing papers in PubMed.
- Of mice and gender: considering gender norms in rodent models.Biology of sex differences · 2026Review
- Systematic Review of Mammalian Models for Experimental Sporotrichosis: Pathogenesis, Methodological Variables, and Ethical Considerations.Animals : an open access journal from MDPI · 2026Review
- Environmental Exposure to the Common Trunk of Mammalian Appeasing Pheromone Modulates Social Behavior and Reduces Fight Wounds in Male Mice.Animals : an open access journal from MDPI · 2025Article
- How Do Individually Ventilated Cages Affect the Welfare of Male BALB/c Mice? Comprehensive Assessment of Behavior, Metabolism, and Responses to Acute Painful Stimuli.Brain and behavior · 2025Article
- Mouse Testing Methods in Psychoneuroimmunology: Measuring Behavioral Responses.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Effects of inter-individual variability in experimenters' sensitivity and training on behavioral assessment of animal models of vestibular disorders.Frontiers in neurology · 2025Article
- A Systematic Review and Meta-Analysis of Anxiety- and Depressive-Like Behaviors in Rodent Models of Neuropathic Pain.Biological psychiatry global open science · 2024Article
- Effects of housing conditions on stress, depressive like behavior and sensory-motor performances of C57BL/6 mice.Laboratory animal research · 2024Article
- Translational models of stress and resilience: An applied neuroscience methodology review.Neuroscience applied · 2024Review
- Experimenter familiarization is a crucial prerequisite for assessing behavioral outcomes and reduces stress in mice not only under chronic pain conditions.Scientific reports · 2023Article
- The rearing environment persistently modulates mouse phenotypes from the molecular to the behavioural level.PLoS biology · 2022Article
- What's wrong with my experiment?: The impact of hidden variables on neuropsychopharmacology research.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2022Review
- Assessing negative affect in mice during abstinence from alcohol drinking: Limitations and future challenges.Alcohol (Fayetteville, N.Y.) · 2022Review
- Evaluation of Various IVC Systems According to Mouse Reproductive Performance and Husbandry and Environmental Parameters.Journal of the American Association for Laboratory Animal Science : JAALAS · 2022Article
- 'Invisible actors'-How poor methodology reporting compromises mouse models of oncology: A cross-sectional survey.PloS one · 2022Article
- Roadbumps at the Crossroads of Integrating Behavioral andFrontiers in toxicology · 2022Review
- Role of Environment and Experimenter in Reproducibility of Behavioral Studies With Laboratory Mice.Frontiers in behavioral neuroscience · 2022Article
- Effect of Estrous Cycle on Behavior of Females in Rodent Tests of Anxiety.Frontiers in psychiatry · 2021Review
- Reproducibility of animal research in light of biological variation.Nature reviews. Neuroscience · 2020Article
- Individual housing of male C57BL/6J mice after weaning impairs growth and predisposes for obesity.PloS one · 2020Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Individually ventilated caging (IVC) systems for rodents are increasingly common in laboratory animal facilities. However, the impact of such substantial change in housing conditions on animal physiology and behavior is still debated. Most importantly, there arise the questions regarding reproducibility and comparison of previous or new phenotypes between the IVC and open cages. The present study was set up for detailed and systematic comparison of behavioral phenotypes in male and female mice of three widely used inbred strains (C57BL/6JRccHsd, DBA/2JRccHsd, 129S2/SvHSd) after being kept in two housing environments (IVC and open cages) for 6 weeks (since 4 weeks of age) before behavioral testing. The tests addressed exploratory, anxiety-like and stress-related behavior (light-dark box, open field, forced swim test, stress-induced hyperthermia), social approach and species-specific behavior (nest building, marble burying). In all tests, large and expected strain differences were found. Somewhat surprisingly, the most striking effect of environment was found for basal body temperature and weight loss after one night of single housing in respective cages. In addition, the performance in light-dark box and open field was affected by environment. Several parameters in different tests showed significant interaction between housing and genetic background. In summary, the IVC housing did not invalidate the well-known differences between the mouse strains which have been established by previous studies. However, within the strains the results can be influenced by sex and housing system depending on the behavioral tasks applied. The bottom-line is that the environmental conditions should be described explicitly in all publications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.