Evidence map›Paper›PMID 30847267›Full record

ArticleOsong public health and research perspectives2019

Specification of Bacteriophage Isolated Against Clinical Methicillin-Resistant

Ahmad Nasser, Reza Azizian, Mohsen Tabasi, Jamil Kheirvari Khezerloo, Fatemah Sadeghpour Heravi, Morovat Taheri Kalani, Norkhoda Sadeghifard, Razieh Amini, Iraj Pakzad, Amin Radmanesh and 1 more

Open access · diamondAbstract read
In one paragraph

Article in Osong public health and research perspectives, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
3.1field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 32 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 6 institutions in 1 country.

Ahmad NasserClinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran.
Reza AzizianClinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran.
Mohsen TabasiLegal Medicine Research Center, Legal Medicine Organization, Tehran, Iran.
Jamil Kheirvari KhezerlooYoung Researchers and Elite Club, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Fatemah Sadeghpour HeraviPertussis Reference Laboratory' Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran.
Morovat Taheri KalaniClinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran.
Norkhoda SadeghifardClinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran.
Razieh AminiDepartment of Molecular Medicine, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Iraj PakzadClinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran.
Amin RadmaneshLegal Medicine Research Center, Legal Medicine Organization, Tehran, Iran.
Farid Azizi JalilianDepartment of Virology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Ilam University · IRHamedan University of Medical Sciences · IRIranian Legal Medicine Organization · IRMedical University of Ilam · IRIslamic Azad University Medical Branch of Tehran · IRPasteur Institute of Iran · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesThe emergence of resistant bacteria is being increasingly reported around the world, potentially threatening millions of lives. Amongst resistant bacteria, methicillin-resistant

methodsIsolation of livestock and sewage bacteriophages were performed using 0.4 μm membrane filters. Plaque assays were used to determine phage quantification by double layer agar method. Pure plaques were then amplified for further characterization. Sulfate-polyacrylamide gel electrophoresis and random amplification of polymorphic DNA were run for protein evaluation, and genotyping respectively. Transmission electron microscope was also used to detect the structure and taxonomic classification of phage visually.

resultsHead and tail morphology of bacteriophages against MRSA were identified by transmission electron microscopy and assigned to the

conclusionBacteriophages are the most abundant microorganism on Earth and coexist with the bacterial population. They can destroy bacterial cells successfully and effectively. They cannot enter mammalian cells which saves the eukaryotic cells from lytic phage activity. In conclusion, phage therapy may have many potential applications in microbiology and human medicine with no side effect on eukaryotic cells.

Indexed as

bacteriophagemethicillin-resistant Staphylococcus aureusrandom amplified polymorphic deoxyribonucleic acid - polymerase chain reactionscanning transmission electron microscopysodium dodecyl sulfate-polyacrylamide gel electrophoresis

Identifiers

PMID30847267
PMCPMC6396822
OpenAlexW2916953987

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.