Evidence map›Paper›PMID 30846481›Full record

ArticleG3 (Bethesda, Md.)2019

Small RNA-Seq Analysis Reveals miRNA Expression Dynamics Across Tissues in the Malaria Vector,

William Bart Bryant, Mary Katherine Mills, Bradley Jsc Olson, Kristin Michel

Open access · goldAbstract read
In one paragraph

Article in G3 (Bethesda, Md.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.6field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 11 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

William Bart BryantDivision of Biology, Kansas State University, Manhattan, KS 66506 and WilliamB@usca.edu.ORCID 0000-0002-3332-4330
Mary Katherine MillsDivision of Biology, Kansas State University, Manhattan, KS 66506 and.ORCID 0000-0002-9483-5986
Bradley Jsc OlsonDivision of Biology, Kansas State University, Manhattan, KS 66506 and.ORCID 0000-0002-4751-8347
Kristin MichelDivision of Biology, Kansas State University, Manhattan, KS 66506 and.ORCID 0000-0001-9769-5248
Kansas State University · USUniversity of South Carolina Aiken · US

Funding

Transgenic and Gener-Targeting CoreP20GM104936 · NIGMS · UNIVERSITY OF KANSAS MEDICAL CENTER · PI SAADI, IRFAN · 2012 to 2016
$10.9M
Using PCORnet to Expand the DS-CONNECT Cohort Through Healthcare System Recruitment, Incorporating Electronic Health Records, and Assessing Self-DeterminationU54HD090216 · NICHD · UNIVERSITY OF KANSAS LAWRENCE · PI COLOMBO, JOHN A. · 2016 to 2020
$6.2M
High Throughput Sequencing System for KUMC Genomics CoreS10OD021743 · OD · UNIVERSITY OF KANSAS MEDICAL CENTER · PI SMITH, PETER G · 2017 to 2017
$493k
Somatic Gene Therapy for MosquitoesR21AI119544 · NIAID · KANSAS STATE UNIVERSITY · PI BRYANT, WILLIAM BART · 2016 to 2017
$407k
Exploring the Role of Ovary MicroRNAs in Mosquito ReproductionR21AI139603 · NIAID · UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA · PI MILLS, MARY KATHERINE · 2018 to 2019
$370k
NIAID NIH HHS R21 AI119544NIAID NIH HHS R21 AI139603NICHD NIH HHS U54 HD090216NIGMS NIH HHS P20 GM104936NIH HHS S10 OD021743
6 · The paper itself

Abstract

Malaria continues to be a major global health problem, where disease transmission is deeply linked to the repeated blood feeding nature of the anautogenous mosquito. Given the tight link between blood feeding and disease transmission, understanding basic biology behind mosquito physiology is a requirement for developing effective vector-borne disease control strategies. In the mosquito, numerous loss of function studies with notable phenotypes demonstrate microRNAs (miRNAs) play significant roles in mosquito physiology. While the field appreciates the importance of a handful of miRNAs, we still need global mosquito tissue miRNA transcriptome studies. To address this need, our goal was to determine the miRNA transcriptome for multiple tissues of the pre-vitellogenic mosquito. To this end, by using small RNA-Seq analysis, we determined miRNA transcriptomes in tissues critical for mosquito reproduction and immunity including (i) fat body-abdominal wall enriched tissues, (ii) midguts, (iii) ovaries, and (iv) remaining tissues comprised of the head and thorax. We found numerous examples of miRNAs exhibiting pan-tissue high- or low- expression, tissue exclusion, and tissue enrichment. We also updated and consolidated the miRNA catalog and provided a detailed genome architecture map for the malaria vector,

Indexed as

Gene Expression ProfilingSequence Analysis, RNATranscriptomeAnimalsAnophelesGene Expression RegulationMalariaMicroRNAsMosquito VectorsOrgan SpecificityMicroRNAsmalariamiRNAmosquito reproductionmosquito tissuesmall RNAs

Identifiers

PMID30846481
PMCPMC6505144
OpenAlexW2921055770

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.