ArticleBiophysical journal2019
SMALL-LABS: Measuring Single-Molecule Intensity and Position in Obscuring Backgrounds.
Article in Biophysical journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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33 citing papers in PubMed.
- Substrate recognition, not sequestration, drives the engagement of an H3K9 methyltransferase in living cells.bioRxiv : the preprint server for biology · 2026Article
- Characterizing the Impact of Nucleoid-Associated Proteins on HU-DNA Interactions by Live-Cell Single-Molecule Tracking.Chemphyschem : a European journal of chemical physics and physical chemistry · 2026Article
- Polyphosphate modulates the stress-responsive formation of functional RNA-protein condensates in bacteria and mammalian cells.PLoS biology · 2026Article
- The kinetics and mobility of a ParA ATPase drive carboxysome distribution inbioRxiv : the preprint server for biology · 2026Article
- Characterizing the Impact of Nucleoid-Associated Proteins on HU-DNA Interactions by Live-Cell Single-Molecule Tracking.bioRxiv : the preprint server for biology · 2026Article
- Dps binds and protects DNA in starved Escherichia coli with minimal effect on chromosome accessibility, dynamics, and organization.Nucleic acids research · 2026Article
- Transient binding facilitates super-resolution imaging of functional amyloid fibrils on living bacteria.Protein science : a publication of the Protein Society · 2026Article
- Dps binds and protects DNA in starvedbioRxiv : the preprint server for biology · 2025Article
- Stress changes the material state of a bacterial biomolecular condensate and shifts its function from mRNA decay to storage.Nature communications · 2025Article
- Genome-wide DNA bridging by H-NS reshapes the stationary phase nucleoid and transcriptional landscape.bioRxiv : the preprint server for biology · 2025Article
- Achiral Plasmonic Antennas Enhance Differential Absorption To Increase Preferential Detection of Chiral Single Molecules.ACS measurement science au · 2024Article
- Tracking live-cell single-molecule dynamics enables measurements of heterochromatin-associated protein-protein interactions.Nucleic acids research · 2024Article
- Epigenetic memory is governed by an effector recruitment specificity toggle in Heterochromatin Protein 1.Nature communications · 2024Article
- Triggered cagedSTORM microscopy.Biomedical optics express · 2024Article
- Near-infrared PAINT localization microscopy via chromophore replenishment of phytochrome-derived fluorescent tag.Communications biology · 2024Article
- An experimental framework to assess biomolecular condensates in bacteria.Nature communications · 2024Article
- Physically informed Monte Carlo simulation of dual-wedge prism-based spectroscopic single-molecule localization microscopy.Journal of biomedical optics · 2024Article
- Tracking live-cell single-molecule dynamics enables measurements of heterochromatinassociated protein-protein interactions.bioRxiv : the preprint server for biology · 2023Article
- An experimental framework to assess biomolecular condensates in bacteria.Research square · 2023Article
- An experimental framework to assess biomolecular condensates in bacteria.bioRxiv : the preprint server for biology · 2023Article
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Authors and funding
4 authors.
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Abstract
Single-molecule and super-resolution imaging relies on successful, sensitive, and accurate detection of the emission from fluorescent molecules. Yet, despite the widespread adoption of super-resolution microscopies, single-molecule data processing algorithms can fail to provide accurate measurements of the brightness and position of molecules in the presence of backgrounds that fluctuate significantly over time and space. Thus, samples or experiments that include obscuring backgrounds can severely, or even completely, hinder this process. To date, no general data analysis approach to this problem has been introduced that is capable of removing obscuring backgrounds for a wide variety of experimental modalities. To address this need, we present the Single-Molecule Accurate LocaLization by LocAl Background Subtraction (SMALL-LABS) algorithm, which can be incorporated into existing single-molecule and super-resolution analysis packages to accurately locate and measure the intensity of single molecules, regardless of the shape or brightness of the background. Accurate background subtraction is enabled by separating the foreground from the background based on differences in the temporal variations of the foreground and the background (i.e., fluorophore blinking, bleaching, or moving). We detail the function of SMALL-LABS here, and we validate the SMALL-LABS algorithm on simulated data as well as real data from single-molecule imaging in living cells.
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