ArticleThe Journal of cell biology1986
Identification of actin-, alpha-actinin-, and vinculin-containing plaques at the lateral membrane of epithelial cells.
Article in The Journal of cell biology, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 139 citations in OpenAlex.
- Visualizing cellular and tissue ultrastructure using Ten-fold Robust Expansion Microscopy (TREx).eLife · 2022Article
- Cadherin puncta are interdigitated dynamic actin protrusions necessary for stable cadherin adhesion.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Structure of the enterocyte transcytosis compartments during lipid absorption.Histochemistry and cell biology · 2020Article
- Myosin-1c promotes E-cadherin tension and force-dependent recruitment of α-actinin to the epithelial cell junction.Journal of cell science · 2018Article
- Cell-cell adhesion interface: rise of the lateral membrane.F1000Research · 2017Review
- Synaptopodin couples epithelial contractility to α-actinin-4-dependent junction maturation.The Journal of cell biology · 2015Article
- Cell adhesion in epidermal development and barrier formation.Current topics in developmental biology · 2015Review
- Arp2/3 complex function in the epidermis.Tissue barriers · 2014Article
- A local, periactive zone endocytic machinery at photoreceptor synapses in close vicinity to synaptic ribbons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- α-Actinin-4/FSGS1 is required for Arp2/3-dependent actin assembly at the adherens junction.The Journal of cell biology · 2012Article
- Adherens junction assembly.Sub-cellular biochemistry · 2012Review
- New insights into vinculin function and regulation.International review of cell and molecular biology · 2011Review
- Accelerators, Brakes, and Gears of Actin Dynamics in Dendritic Spines.The open neuroscience journal · 2009Article
- Molecular components of the adherens junction.Biochimica et biophysica acta · 2008Review
- Glucocorticoid effects on mouse microvascular endothelial barrier permeability are brain specific.The Journal of physiology · 2006Article
- Immunocytochemical characterization of two types of microvillar cells in rodent olfactory epithelium.Histochemistry and cell biology · 2005Article
- Comparison of the small heat shock proteins alphaB-crystallin, MKBP, HSP25, HSP20, and cvHSP in heart and skeletal muscle.Histochemistry and cell biology · 2004Article
- The nucleoporin Nup98 associates with the intranuclear filamentous protein network of TPR.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Cellular and regional distribution of the glutamate transporter GLAST in the CNS of rats: nonradioactive in situ hybridization and comparative immunocytochemistry.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
- Cytoskeletal markers allowing discrimination between brush cells and other epithelial cells of the gut including enteroendocrine cells.Histochemistry and cell biology · 1996Article
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this paper, a new type of spot desmosome-like junction (type II plaque) is described that is scattered along the entire lateral plasma membrane of rat and human intestinal epithelium. Ultrastructurally type II plaques differed from the classical type of epithelial spot desmosome ("macula adherens", further denoted as type I desmosome) by weak electron density of the membrane-associated plaque material, association of the plaques with microfilaments rather than intermediate filaments, and poorly visible material across the intercellular space. Thus, type II plaques resemble cross-sections of the zonula adherens. Immunofluorescence-microscopic studies were done using antibodies to a main protein associated with the plaques of type I desmosomes (desmoplakin I) and to the three major proteins located at the plaques of the zonula adherens (actin, alpha-actinin, and vinculin). Two types of plaques were visualized along the lateral surface of intestinal and prostatic epithelium: (a) the type I desmosomes, which were labeled with anti-desmoplakin but did not bind antibodies to actin, alpha-actinin, and vinculin, and (b) a further set of similarly sized plaques, which bound antibodies to actin, alpha-actinin, and vinculin but were not stained with anti-desmoplakin. Three-dimensional computer reconstruction of serial sections double-labeled with anti-desmoplakin and anti-alpha-actinin further confirmed that both types of plaques are spatially completely separated from each other along the lateral plasma membrane. The computer graphs further revealed that the actin-, alpha-actinin-, and vinculin-containing plaques have the tendency to form clusters, a feature also typical of type II plaques. It is suggested that the type II plaques represent spot desmosome-like intercellular junctions, which, like the zonula adherens, appear to be linked to the actin filament system. As the type II plaques cover a considerable part of the lateral cell surface, they might play a particular role in controlling cellular shape and intercellular adhesion.
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