ArticleThe Journal of cell biology1986
Changes in membrane-microfilament interaction in intercellular adherens junctions upon removal of extracellular Ca2+ ions.
Article in The Journal of cell biology, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
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Who cites it
69 citing papers in PubMed, 227 citations in OpenAlex.
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- Actin cytoskeleton self-organization in single epithelial cells and fibroblasts under isotropic confinement.Journal of cell science · 2019Article
- Gammaherpesvirus BoHV-4 infects bovine respiratory epithelial cells mainly at the basolateral side.Veterinary research · 2019Article
- Cell force-mediated matrix reorganization underlies multicellular network assembly.Scientific reports · 2019Article
- Access to a main alphaherpesvirus receptor, located basolaterally in the respiratory epithelium, is masked by intercellular junctions.Scientific reports · 2017Article
- Quantifying cadherin mechanotransduction machinery assembly/disassembly dynamics using fluorescence covariance analysis.Scientific reports · 2016Article
- Balancing spatially regulated β-actin translation and dynamin-mediated endocytosis is required to assemble functional epithelial monolayers.Cytoskeleton (Hoboken, N.J.) · 2015Article
- c-Jun N-terminal kinase inhibitor SP600125 enhances barrier function and elongation of human pancreatic cancer cell line HPAC in a Ca-switch model.Histochemistry and cell biology · 2015Article
- The β-actin mRNA zipcode regulates epithelial adherens junction assembly but not maintenance.RNA (New York, N.Y.) · 2014Article
- Vinculin phosphorylation differentially regulates mechanotransduction at cell-cell and cell-matrix adhesions.The Journal of cell biology · 2014Article
- Development and remodeling of the vertebrate blood-gas barrier.BioMed research international · 2013Review
- LI-cadherin cis-dimerizes in the plasma membrane Ca(2+) independently and forms highly dynamic trans-contacts.Cellular and molecular life sciences : CMLS · 2012Article
- Galectin-3 protein regulates mobility of N-cadherin and GM1 ganglioside at cell-cell junctions of mammary carcinoma cells.The Journal of biological chemistry · 2012Article
- Nonredundant roles of cytoplasmic β- and γ-actin isoforms in regulation of epithelial apical junctions.Molecular biology of the cell · 2012Article
- Article
- Potential of human umbilical cord blood mesenchymal stem cells to heal damaged corneal endothelium.Molecular vision · 2012Article
- The small GTPase Rap1 is a novel regulator of RPE cell barrier function.Investigative ophthalmology & visual science · 2011Article
- Spectrin-adducin membrane skeleton: A missing link between epithelial junctions and the actin cytoskeletion?Bioarchitecture · 2011Article
- Enhancement of drug absorption through the blood-brain barrier and inhibition of intercellular tight junction resealing by E-cadherin peptides.Molecular pharmaceutics · 2011Article
9 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
EGTA-induced depletion of Ca2+ ions from the culture medium of Madin-Darby bovine kidney epithelial cells results in rapid splitting of adherens-type junctions and the detachment of the vinculin- and actin-containing filament bundle from the cytoplasmic faces of the plasma membrane of the zonula adhaerens. This process was monitored by phase-contrast microscopy, combined with electron microscopy and immunofluorescent localization of the two proteins. It is shown that shortly after extracellular free Ca2+ concentration is lowered to the micromolar range, the actin-containing, junction-associated belt of microfilaments, together with the vinculin-rich junctional plaque material, is irreversibly detached as one structural unit from the plasma membrane, contracts, and is displaced towards the perinuclear cytoplasm where it gradually disintegrates. Other actin- and vinculin-containing structures present in the same cells, notably the focal contacts at the substratum, are not similarly affected by the Ca2+ depletion and retain both the adhesion to the external surface and the association with the plaque and microfilament components. Electron microscopic examination has shown that the membrane domain of the zonulae adhaerentes, unlike that of desmosomes, is not endocytosed after Ca2+ removal and that the displaced actin- and vinculin-containing plaque and filament belt are not associated with a particular membrane. It is further shown that upon restoration of normal Ca2+ levels in the culture medium, new intercellular contacts are established gradually by accretion of both vinculin and actin into new belt-like plaque- and microfilament-containing structures.
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