ArticleThe Journal of antimicrobial chemotherapy2019
Using WGS to identify antibiotic resistance genes and predict antimicrobial resistance phenotypes in MDR Acinetobacter baumannii in Tanzania.
Article in The Journal of antimicrobial chemotherapy, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 66 citations in OpenAlex.
- Genomic characterization of clinical isolates of carbapenem-resistantJAC-antimicrobial resistance · 2026Article
- Determinants of Antimicrobial Resistance inInternational journal of microbiology · 2026Article
- SeqAfrica: empowering Africa's fight against antimicrobial resistance through genomics.Frontiers in public health · 2025Article
- The burden, risk factors, and antimicrobial susceptibility pattern associated with extended-spectrum beta-lactamase-producingFrontiers in antibiotics · 2025Article
- Neural network-based predictions of antimicrobial resistance phenotypes in multidrug-resistantAntimicrobial agents and chemotherapy · 2024Article
- ESKAPE pathogens: antimicrobial resistance, epidemiology, clinical impact and therapeutics.Nature reviews. Microbiology · 2024Review
- Whole-genome sequencing of two multidrug-resistant acinetobacter baumannii strains isolated from a neonatal intensive care unit in Egypt: a prospective cross-sectional study.BMC microbiology · 2024Article
- Comprehensive Genome Analysis of Colistin-Only-Sensitive KPC-2 and NDM1-1-CoproducingInternational journal of microbiology · 2024Article
- Unveiling Rare Pathogens and Antibiotic Resistance in Tanzanian Cholera Outbreak Waters.Microorganisms · 2023Article
- CRISPR-Based Gene Editing inPharmaceuticals (Basel, Switzerland) · 2023Review
- Novel Clinical mNGS-Based Machine Learning Model for Rapid Antimicrobial Susceptibility Testing of Acinetobacter baumannii.Journal of clinical microbiology · 2023Article
- Antimicrobial Resistance in Romania: Updates on Gram-Negative ESCAPE Pathogens in the Clinical, Veterinary, and Aquatic Sectors.International journal of molecular sciences · 2023Review
- Clinical Diagnostics of Bacterial Infections and Their Resistance to Antibiotics-Current State and Whole Genome Sequencing Implementation Perspectives.Antibiotics (Basel, Switzerland) · 2023Review
- Metagenomic profiling of antibiotic resistance genes in Red Sea brine pools.Archives of microbiology · 2023Article
- Outpatient Clonal Propagation and Rapid Regional Establishment of an Emergent Carbapenem-Resistant Acinetobacter baumannnii Lineage Sequence Type 499Pas.The Journal of infectious diseases · 2023Article
- Analysis of Antimicrobial Resistance Genes (ARGs) in Enterobacterales andAntibiotics (Basel, Switzerland) · 2023Article
- Molecular epidemiology and antimicrobial resistance patterns of carbapenem-resistantFrontiers in cellular and infection microbiology · 2023Article
- Antimicrobial resistance genes ofAIMS microbiology · 2023Review
- Whole-Genome Sequencing of ST2Antibiotics (Basel, Switzerland) · 2022Article
- Article
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundReliable phenotypic antimicrobial susceptibility testing can be a challenge in clinical settings in low- and middle-income countries. WGS is a promising approach to enhance current capabilities.
aimTo study diversity and resistance determinants and to predict and compare resistance patterns from WGS data of Acinetobacter baumannii with phenotypic results from classical microbiological testing at a tertiary care hospital in Tanzania. METHODS AND
resultsMLST using Pasteur/Oxford schemes yielded eight different STs from each scheme. Of the eight, two STs were identified to be global clones 1 (n = 4) and 2 (n = 1) as per the Pasteur scheme. Resistance testing using classical microbiology determined between 50% and 92.9% resistance across all drugs. Percentage agreement between phenotypic and genotypic prediction of resistance ranged between 57.1% and 100%, with coefficient of agreement (κ) between 0.05 and 1. Seven isolates harboured mutations at significant loci (S81L in gyrA and S84L in parC). A number of novel plasmids were detected, including pKCRI-309C-1 (219000 bp) carrying 10 resistance genes, pKCRI-43-1 (34935 bp) carrying two resistance genes and pKCRI-49-1 (11681 bp) and pKCRI-28-1 (29606 bp), each carrying three resistance genes. New ampC alleles detected included ampC-69, ampC-70 and ampC-71. Global clone 1 and 2 isolates were found to harbour ISAba1 directly upstream of the ampC gene. Finally, SNP-based phylogenetic analysis of the A. baumannii isolates revealed closely related isolates in three clusters.
conclusionsThe validity of the use of WGS in the prediction of phenotypic resistance can be appreciated, but at this stage is not sufficient for it to replace conventional antimicrobial susceptibility testing in our setting.
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