ArticleScientific reports2019
Proteomic changes in oocytes after in vitro maturation in lipotoxic conditions are different from those in cumulus cells.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 50 citations in OpenAlex.
- Human follicular fluid shows diverse metabolic profiles at different follicle developmental stages.Reproductive biology and endocrinology : RB&E · 2020Trial
- Effects of HMG on Superovulation Efficacy in Kazakh Sheep and Screening of Plasma Reproductive Hormones and Metabolic Markers.Animals : an open access journal from MDPI · 2026Article
- Agomelatine alleviates palmitic acid-induced mouse oocyte meiosis defects by restoring mitochondrial function.International journal of obesity (2005) · 2025Article
- Increased apoptosis in late-developing in vitro fertilized bovine blastocysts decreases successful pregnancy.Animal bioscience · 2025Article
- Delineating lipidomic landscapes in human and mouse ovaries: Spatial signatures and chemically-induced alterations via MALDI mass spectrometry imaging: Spatial ovarian lipidomics.Environment international · 2024Article
- Evaluation of Lipids and Lipid-Related Transcripts in Human and Ovine Theca Cells and anEnvironmental health perspectives · 2024Article
- Preconception Diet Interventions in Obese Outbred Mice and the Impact on Female Offspring Metabolic Health and Oocyte Quality.International journal of molecular sciences · 2024Article
- Risks of using mitoquinone during in vitro maturation and its potential protective effects against lipotoxicity-induced oocyte mitochondrial stress.Journal of assisted reproduction and genetics · 2024Article
- Effect of lipotoxicity on mitochondrial function and epigenetic programming during bovine in vitro embryo production.Scientific reports · 2023Article
- miR-202-5p Inhibits Lipid Metabolism and Steroidogenesis of Goose Hierarchical Granulosa Cells by Targeting ACSL3.Animals : an open access journal from MDPI · 2023Article
- High-resolution ribosome profiling reveals translational selectivity for transcripts in bovine preimplantation embryo development.Development (Cambridge, England) · 2022Article
- Effects of early lactation body condition loss in dairy cows on serum lipid profiles and on oocyte and cumulus cell transcriptomes.Journal of dairy science · 2022Article
- Cellular Stress Responses in Oocytes: Molecular Changes and Clinical Implications.Advances in experimental medicine and biology · 2022Review
- Uncovering the important role of mitochondrial dynamics in oogenesis: impact on fertility and metabolic disorder transmission.Biophysical reviews · 2021Review
- Diet normalization or caloric restriction as a preconception care strategy to improve metabolic health and oocyte quality in obese outbred mice.Reproductive biology and endocrinology : RB&E · 2021Article
- Protein Palmitoylation in Bovine Ovarian Follicle.International journal of molecular sciences · 2021Article
- Proteomic Exploration of Porcine Oocytes During Meiotic MaturationFrontiers in veterinary science · 2021Article
- Rescue Potential of Supportive Embryo Culture Conditions on Bovine Embryos Derived from Metabolically Compromised Oocytes.International journal of molecular sciences · 2020Article
- Differential effects of high fat diet-induced obesity on oocyte mitochondrial functions in inbred and outbred mice.Scientific reports · 2020Article
- Non-esterified fatty acids in the ovary: friends or foes?Reproductive biology and endocrinology : RB&E · 2020Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Maternal lipolytic metabolic disorders result in a lipotoxic microenvironment in the ovarian follicular fluid (FF) which deteriorates oocyte quality. Although cellular stress response mechanisms are well defined in somatic cells, they remain largely unexplored in oocytes, which have distinct organelle structure and nuclear transcription patterns. Here we used shotgun proteomic analyses to study cellular responses of bovine oocytes and cumulus cells (CCs) after in vitro maturation under lipotoxic conditions; in the presence of pathophysiological palmitic acid (PA) concentration as a model. Differentially regulated proteins (DRPs) were mainly localized in the endoplasmic reticulum, mitochondria and nuclei of CCs and oocytes, however the DRPs and their direction of change were cell-type specific. Proteomic changes in PA-exposed CCs were predominantly pro-apoptotic unfolded protein responses (UPRs), mitochondrial and metabolic dysfunctions, and apoptotic pathways. This was also functionally confirmed. Interestingly, although the oocytes were enclosed by CCs during PA exposure, elevated cellular stress levels were also evident. However, pro-survival UPRs, redox regulatory and compensatory metabolic mechanisms were prominent despite evidence of mitochondrial dysfunction, oxidative stress, and reduced subsequent embryo development. The data provides a unique insight that enriches the understanding of the cellular stress responses in metabolically-compromised oocytes and forms a fundamental base to identify new targets for fertility treatments as discussed within.
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