ArticleScientific reports2019
Pyruvate-depleting conditions induce biofilm dispersion and enhance the efficacy of antibiotics in killing biofilms in vitro and in vivo.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers, 1 of them a synthesis that pooled it.
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Who cites it
54 citing papers in PubMed, 1 synthesis or guideline pooled it, 80 citations in OpenAlex.
- Reducing Biofilm Infections in Burn Patients' Wounds and Biofilms on Surfaces in Hospitals, Medical Facilities and Medical Equipment to Improve Burn Care: A Systematic Review.International journal of environmental research and public health · 2021Pooled it
- Article
- Bacteriophages in the Rhizosphere: Roles in Nutrient Cycling, Bacterial Community Structure, and Animal-Mediated Dispersal.MicrobiologyOpen · 2026Review
- Fragmented but functional: Post-dispersion dynamics and phenotypic variation in dispersed biofilm-associated cells.Biofilm · 2026Article
- Biofilm dispersion inbioRxiv : the preprint server for biology · 2026Article
- Assessment of the effect of culture components of clinical Pseudomonas aeruginosa isolates on vancomycin eradication of Staphylococcus aureus mature biofilms.BMC research notes · 2026Article
- Rapid means of biofilm disruption induce the newly released (NRel) phenotype of enhanced antibiotic sensitivity.Frontiers in microbiology · 2026Article
- Characterization ofFrontiers in microbiology · 2026Article
- α-Amylase-Mediated Antibiotic Degradation and Sequestration inAntibiotics (Basel, Switzerland) · 2025Article
- Molecular characterization and inhibition of cyclic-di-GMP receptor proteins inBiochemistry and biophysics reports · 2025Article
- Respiratory tract antimicrobial peptides more effectively killed multiple methicillin-resistantMicrobiology spectrum · 2025Article
- The Effect of the Ratio of Butylene Succinate and Dilinoleic Diol in Their Copolyester (PBS-DLS) on the Physicochemical Properties and Biofilm Formation.Molecules (Basel, Switzerland) · 2025Article
- An engineered peptide derived from the innate immune effector high-mobility group box 1 disrupts and prevents dual-genera biofilms formed by common respiratory tract pathogens.FEMS microbiology letters · 2025Article
- Therapeutic Potential of Emodin: A Mini-Review of Its Anti-Biofilm and Antimicrobial Effects.Infectious disorders drug targets · 2025Review
- Human PMNs exhibit greater engulfment, NETosis, and enhanced migration when incubated with nontypeableFrontiers in microbiology · 2025Article
- Unraveling metabolic reprogramming inFrontiers in molecular biosciences · 2025Article
- H-NOX Influences Biofilm Formation, Central Metabolism, and Quorum Sensing inJournal of proteome research · 2024Article
- Untangling the adaptive strategies of thermophilic bacterium Anoxybacillus rupiensis TPH1 under low temperature.Extremophiles : life under extreme conditions · 2024Article
- Nanoparticle-Based Strategies for Managing Biofilm Infections in Wounds: A Comprehensive Review.ACS omega · 2024Review
- Quorum-quenching enzyme Est816 assisted antibiotics against periodontitis induced byFrontiers in cellular and infection microbiology · 2024Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
The formation of biofilms is a developmental process initiated by planktonic cells transitioning to the surface, which comes full circle when cells disperse from the biofilm and transition to the planktonic mode of growth. Considering that pyruvate has been previously demonstrated to be required for the formation of P. aeruginosa biofilms, we asked whether pyruvate likewise contributes to the maintenance of the biofilm structure, with depletion of pyruvate resulting in dispersion. Here, we demonstrate that the enzymatic depletion of pyruvate coincided with the dispersion of established biofilms by S. aureus and laboratory and clinical P. aeruginosa isolates. The dispersion response was dependent on pyruvate fermentation pathway components but independent of proteins previously described to contribute to P. aeruginosa biofilm dispersion. Using porcine second-degree burn wounds infected with P. aeruginosa biofilm cells, we furthermore demonstrated that pyruvate depletion resulted in a reduction of biofilm biomass in vivo. Pyruvate-depleting conditions enhanced the efficacy of tobramycin killing of the resident wound biofilms by up to 5-logs. Our findings strongly suggest the management of pyruvate availability to be a promising strategy to combat biofilm-related infections by two principal pathogens associated with wound and cystic fibrosis lung infections.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.