ArticleScientific reports2019
Xist/Tsix expression dynamics during mouse peri-implantation development revealed by whole-mount 3D RNA-FISH.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 31 citations in OpenAlex.
- Antisense transcription can induce expression memory via stable promoter repression.Genome biology · 2025Article
- Reporter CRISPR screens decipher cis-regulatory and trans-regulatory principles at the Xist locus.Nature structural & molecular biology · 2025Article
- The Transgenerational Impact of High-Fat Diet and Diabetic Pregnancy on Embryonic Transcriptomics and Mitochondrial Health.Biomedicines · 2025Article
- Transcriptional stochasticity reveals multiple mechanisms of long non-coding RNA regulation at the Xist-Tsix locus.Nature communications · 2025Article
- Stepwise de novo establishment of inactive X chromosome architecture in early development.Nature genetics · 2024Article
- Establishment and maintenance of random monoallelic expression.Development (Cambridge, England) · 2024Review
- Hallmarks of totipotent and pluripotent stem cell states.Cell stem cell · 2024Review
- Review
- GATA transcription factors drive initial Xist upregulation after fertilization through direct activation of long-range enhancers.Nature cell biology · 2023Article
- iSMOD: an integrative browser for image-based single-cell multi-omics data.Nucleic acids research · 2023Article
- Regulatory principles and mechanisms governing the onset of random X-chromosome inactivation.Current opinion in genetics & development · 2023Review
- Stepwise pluripotency transitions in mouse stem cells.EMBO reports · 2022Review
- Preventing erosion of X-chromosome inactivation in human embryonic stem cells.Nature communications · 2022Article
- The role of lncRNAs andFrontiers in cell and developmental biology · 2022Review
- Capture of Mouse and Human Stem Cells with Features of Formative Pluripotency.Cell stem cell · 2021Article
- The Non-random Location of Autosomal Genes That Participate in X Inactivation.Frontiers in cell and developmental biology · 2019Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During peri-implantation development in mice, X chromosome inactivation (XCI) status changes dynamically. Here, we examined the expression of Xist and its antisense partner, Tsix, via whole-mount 3D RNA-FISH using strand-specific probes and evaluated XCI status. The results indicate that Xist expression disappears completely by embryonic day (E) 4.5 without Tsix activation in the ICM and that Xist re-expression occurs at E4.75 in some cells, suggesting that random XCI is already initiated in these cells. Intriguingly, epiblast cells exhibiting biallelic Xist expression were observed frequently (~15%) at E5.25 and E5.5. Immunostaining analysis of epigenetic modifications suggests that global change in epigenomic status occurs concomitantly with the transition from imprinted to random XCI. However, global upregulation of H3K27me3 modifications initiated earlier than other modifications, occurring specifically in ICM during progression of Xist erasure. Although both Xist expression and imprinted XCI are thought to be stable in the primitive endoderm/visceral endoderm and trophectoderm/extraembryonic ectoderm lineages, transient loss of Xist clouds was noted only in a subset of extraembryonic ectodermal cells, suggesting distinct features of Xist regulation among the three different embryonic tissue layers. These results will serve as a basis for future functional studies of XCI regulation in vivo.
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