ArticleBMC developmental biology2019
Computational fluid dynamic analysis of physical forces playing a role in brain organoid cultures in two different multiplex platforms.
Article in BMC developmental biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 2 of them syntheses that pooled it.
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Who cites it
29 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Mapping the Cerebral Organoid Landscape: A Systematic Review of Preclinical 3D Models in Neuroscience.Advanced healthcare materials · 2026Pooled it
- Recent advancements and future requirements in vascularization of cortical organoids.Frontiers in bioengineering and biotechnology · 2022Pooled it
- Microcephaly-like phenotype triggered by novel reassortant and prototypic Oropouche virus strains in brain organoids.EBioMedicine · 2026Article
- Genetically engineered human cell-based microrobots for selective cancer cell death.Science advances · 2026Article
- A modular platform for automated organoid culture and longitudinal imaging.Scientific reports · 2026Article
- Article
- Brain organoids: building higher-order complexity and neural circuitry models.Trends in biotechnology · 2025Review
- Article
- Dynamic culture of cerebral organoids using a pillar/perfusion plate for the assessment of developmental neurotoxicity.Biofabrication · 2024Article
- Saxitoxin potentiates human neuronal cell death induced by Zika virus while sparing neural progenitors and astrocytes.Scientific reports · 2024Article
- LSD Modulates Proteins Involved in Cell Proteostasis, Energy Metabolism and Neuroplasticity in Human Cerebral Organoids.ACS omega · 2024Article
- Diving into the proteomic atlas of SARS-CoV-2 infected cells.Scientific reports · 2024Article
- Dynamic culture of cerebral organoids using a pillar/perfusion plate for the assessment of developmental neurotoxicity.bioRxiv : the preprint server for biology · 2024Article
- A miniaturized culture platform for control of the metabolic environment.Biomicrofluidics · 2024Article
- Brain organoids: A revolutionary tool for modeling neurological disorders and development of therapeutics.Biotechnology and bioengineering · 2024Review
- Bioreactor Technologies for Enhanced Organoid Culture.International journal of molecular sciences · 2023Review
- Spatio-temporal dynamics enhance cellular diversity, neuronal function and further maturation of human cerebral organoids.Communications biology · 2023Article
- Modular automated microfluidic cell culture platform reduces glycolytic stress in cerebral cortex organoids.Scientific reports · 2022Article
- Human Brain Organoids-on-Chip: Advances, Challenges, and Perspectives for Preclinical Applications.Pharmaceutics · 2022Review
- Age-linked suppression of lipoxin A4 associates with cognitive deficits in mice and humans.Translational psychiatry · 2022Article
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOrganoid cultivation in suspension culture requires agitation at low shear stress to allow for nutrient diffusion, which preserves tissue structure. Multiplex systems for organoid cultivation have been proposed, but whether they meet similar shear stress parameters as the regularly used spinner flask and its correlation with the successful generation of brain organoids has not been determined.
resultsHere we used computational fluid dynamics (CFD) to simulate two multiplex culture conditions: steering plates on an orbital shaker and the use of a previously described bioreactor. The bioreactor had low speed and high shear stress regions that may affect cell aggregate growth, depending on volume, whereas the computed variables of the steering plates were closer to those of the spinning flask.
conclusionOur protocol improves the initial steps of the standard brain organoid formation, and the produced organoids displayed regionalized brain structures, including retinal pigmented cells. Overall, we conclude that suspension culture on orbital steering plates is a cost-effective practical alternative to previously described platforms for the cultivation of brain organoids for research and multiplex testing.
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