Evidence map›Paper›PMID 30841911›Full record

ArticleReproductive biology and endocrinology : RB&E2019

Seasonal variation in equine follicular fluid proteome.

G A Dutra, G M Ishak, O Pechanova, T Pechan, D G Peterson, J C F Jacob, S T Willard, P L Ryan, E L Gastal, J M Feugang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Reproductive biology and endocrinology : RB&E, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
4.5field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 20 citations in OpenAlex.

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  11. Proteome changes of porcine follicular fluid during follicle development.Journal of animal science and biotechnology · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 3 countries.

G A DutraDepartment of Animal Science, Food and Nutrition, Southern Illinois University, Carbondale, IL, USA.
G M IshakDepartment of Animal Science, Food and Nutrition, Southern Illinois University, Carbondale, IL, USA.
O PechanovaInstitute for Genomics, Biocomputing and Bioinformatics, University, Mississippi State, Oxford, MS, USA.
T PechanInstitute for Genomics, Biocomputing and Bioinformatics, University, Mississippi State, Oxford, MS, USA.
D G PetersonInstitute for Genomics, Biocomputing and Bioinformatics, University, Mississippi State, Oxford, MS, USA.
J C F JacobDepartment of Reproduction and Animal Evaluation, Federal Rural University of Rio de Janeiro, Seropédica, Brazil.
S T WillardDepartment of Animal and Dairy Sciences, Mississippi State University, Mississippi State, 4025 Wise Center, PO Box 9815, Mississippi State, MS, 39762, USA.
P L RyanDepartment of Animal and Dairy Sciences, Mississippi State University, Mississippi State, 4025 Wise Center, PO Box 9815, Mississippi State, MS, 39762, USA.
E L GastalDepartment of Animal Science, Food and Nutrition, Southern Illinois University, Carbondale, IL, USA.
J M FeugangDepartment of Animal and Dairy Sciences, Mississippi State University, Mississippi State, 4025 Wise Center, PO Box 9815, Mississippi State, MS, 39762, USA. jn181@ads.msstate.edu.
Biocom · USMississippi State University · USUniversidade Federal Rural do Rio de Janeiro · BRSouthern Illinois University Carbondale · USUniversity of Baghdad · IQ

Funding

Training and Mentoring Core P20GM103476 · NIGMS · UNIVERSITY OF SOUTHERN MISSISSIPPI · PI MICHAEL R GARRETT · 2012 to 2026
$60.2M
National Institutes of Health 4P20GM103476-15NIGMS NIH HHS P20 GM103476USDA-ARS 8-6402-3-018
6 · The paper itself

Abstract

backgroundProteomic studies of follicular fluid (FF) exist for several species, including the horse; however, the seasonal influence on FF proteome has not been explored in livestock. The application of high-throughput proteomics of FF in horse has the potential to identify seasonal variations of proteins involved in follicle and oocyte growth.

methodsThis study (i) profiles the proteomes of equine FF collected from dominant growing follicles during the spring anovulatory season (SAN), and spring (SOV), summer (SUM), and fall (FOV) ovulatory seasons; and (ii) identifies season-dependent regulatory networks and associated key proteins.

resultsRegardless of season, a total of 90 proteins were identified in FF, corresponding to 63, 72, 69, and 78 proteins detected in the SAN, SOV, SUM, and FOV seasons, respectively. Fifty-two proteins were common to all seasons, a total of 13 were unique to either season, and 25 were shared between two seasons or more. Protein-to-protein interaction (PPI) analysis indicated the likely critical roles of plasminogen in the SAN season, the prothrombin/plasminogen combination in SUM, and plasminogen/complement C3 in both SOV and FOV seasons. The apolipoprotein A1 appeared crucial in all seasons. The present findings show that FF proteome of SUM differs from other seasons, with FF having high fluidity (low viscosity).

conclusionsThe balance between the FF contents in prothrombin, plasminogen, and coagulation factor XII proteins favoring FF fluidity may be crucial at the peak of the ovulatory season (SUM) and may explain the reported lower incidence of hemorrhagic anovulatory follicles during the SUM season.

Indexed as

AnimalsFemaleFollicular FluidHorsesProteinsProteomicsReproductionSeasonsProteinsFollicleMares, horsesOvaryProteomicsSeasonality

Identifiers

PMID30841911
PMCPMC6404268
OpenAlexW2936994676

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.