Evidence map›Paper›PMID 30841883›Full record

ArticleBMC veterinary research2019

Development and application of a baculovirus-expressed capsid protein-based indirect ELISA for detection of porcine circovirus 3 IgG antibodies.

Sujiao Zhang, Dongliang Wang, Yifan Jiang, Zhoumian Li, Yawen Zou, Meng Li, Haoyang Yu, Kun Huang, Yi Yang, Naidong Wang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
3.2field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 36 citations in OpenAlex.

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  12. Porcine circovirus 3: a new challenge to explore.Frontiers in veterinary science · 2023
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Sujiao ZhangHunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Laboratory of Functional Proteomics, Research Center of Reverse Vaccinology, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China.
Dongliang WangHunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Laboratory of Functional Proteomics, Research Center of Reverse Vaccinology, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China.
Yifan JiangHunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Laboratory of Functional Proteomics, Research Center of Reverse Vaccinology, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China.
Zhoumian LiHunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Laboratory of Functional Proteomics, Research Center of Reverse Vaccinology, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China.
Yawen ZouHunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Laboratory of Functional Proteomics, Research Center of Reverse Vaccinology, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China.
Meng LiHunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Laboratory of Functional Proteomics, Research Center of Reverse Vaccinology, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China.
Haoyang YuHunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Laboratory of Functional Proteomics, Research Center of Reverse Vaccinology, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China.
Kun HuangHunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Laboratory of Functional Proteomics, Research Center of Reverse Vaccinology, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China.
Yi YangHunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Laboratory of Functional Proteomics, Research Center of Reverse Vaccinology, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China.
Naidong WangHunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Laboratory of Functional Proteomics, Research Center of Reverse Vaccinology, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China. naidongwang@hunau.edu.cn.ORCID http://orcid.org/0000-0001-6905-7767
Hunan Agricultural University · CN

Funding

Hunan Provincial Natural Science Foundation of China 2018JJ2177Postgraduate Research and Innovation Project of Hunan Province CX2016B285 and CX2016B314Research Foundation of Hunan Provincial Education Department, China 15A086
6 · The paper itself

Abstract

backgroundPorcine circovirus type 3 (PCV3), recently widely isolated from pigs with various clinical conditions, is likely globally epidemic. However, development of serological diagnosis for PCV3 in pigs is ongoing. Our objectives were to: 1) establish an indirect ELISA, using PCV3 capsid protein (Cap) prepared by Baculovirus Expression Vector System (BEVS) as a high-quality coating antigen for detection of PCV3-associated antibodies in serum samples; and 2) use this ELISA to conduct a serological survey for PCV3 in various regions of Hunan province, China.

resultsThe PCV3 positive rate to the ELISA assay (total of 190 serum samples) was higher in sows with reproductive failure compared to healthy sows (34/85, 40.0% versus 30/105, 28.6%), with similar results using qPCR assays. Further, in an additional 1038 serum samples collected from January 2016 to May 2018 in various regions of Hunan province and tested with this established ELISA, 20 to 84% were positive for PCV3 (according to region of sera collection), with high PCV3 seroprevalence (> 50%) in herds in Changde, Hengyang and Yueyang. Moreover, among serum samples from herds in Shaoyang and Changde, PCV3 seroprevalence was higher in sows than in other classes of pigs (i.e., suckling piglets, nursery pigs, gilts, growing-finishing pigs and boars).

conclusionsWe developed a full-length PCV3 Cap-based ELISA using a eukaryotic expression system with excellent potential to elucidate PCV3 epidemiology. Based on this assay, PCV3 has been circulating in Hunan province. PCV3 prevalence was lower in healthy sows than in those with reproductive failure. Further studies are warranted to identify the PCV3 responsible for high seroprevalence in sows and determine pathogenesis of PCV3 in sows with reproductive failure.

Indexed as

AnimalsBaculoviridaeCapsid ProteinsChinaCircoviridae InfectionsCircovirusEnzyme-Linked Immunosorbent AssayFemaleGenitalia, FemaleImmunoglobulin GMaleSeroepidemiologic StudiesSwineSwine DiseasesCapsid ProteinsImmunoglobulin GDiagnosticELISAPigPorcine circovirus type 3

Identifiers

PMID30841883
PMCPMC6404275
OpenAlexW2942862892

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.