Evidence map›Paper›PMID 30839645›Full record

ArticleLight, science & applications2018

Label-free 3D computational imaging of spermatozoon locomotion, head spin and flagellum beating over a large volume.

Mustafa Ugur Daloglu, Wei Luo, Faizan Shabbir, Francis Lin, Kevin Kim, Inje Lee, Jia-Qi Jiang, Wen-Jun Cai, Vishwajith Ramesh, Meng-Yuan Yu and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Light, science & applications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed.

  1. Article
  2. Article
  3. Observational
  4. Article
  5. Article
  6. Review
  7. Structural Analysis of Sperm Centrioles Using N-STORM.Methods in molecular biology (Clifton, N.J.) · 2024
    Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Review
  13. CatSper and its CaM-like CaFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022
    Article
  14. Article
  15. Review
  16. Article
  17. Article
  18. Machine learning for sperm selection.Nature reviews. Urology · 2021
    Review
  19. Modelling Motility: The Mathematics of Spermatozoa.Frontiers in cell and developmental biology · 2021
    Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Mustafa Ugur DalogluElectrical and Computer Engineering Department, University of California, Los Angeles, CA 90095, USA.
Wei LuoElectrical and Computer Engineering Department, University of California, Los Angeles, CA 90095, USA.
Faizan ShabbirElectrical and Computer Engineering Department, University of California, Los Angeles, CA 90095, USA.
Francis LinBioengineering Department, University of California, Los Angeles, CA 90095, USA.
Kevin KimChemistry and Biochemistry Department, University of California, Los Angeles, CA 90095, USA.
Inje LeeBioengineering Department, University of California, Los Angeles, CA 90095, USA.
Jia-Qi JiangDepartment of Physics and Astronomy, University of California, Los Angeles, CA 90095, USA.
Wen-Jun CaiDepartment of Mathematics, University of California, Los Angeles, CA 90095, USA.
Vishwajith RameshBioengineering Department, University of California, Los Angeles, CA 90095, USA.
Meng-Yuan YuComputer Science Department, University of California, Los Angeles, CA 90095, USA.
Aydogan OzcanElectrical and Computer Engineering Department, University of California, Los Angeles, CA 90095, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We report a high-throughput and label-free computational imaging technique that simultaneously measures in three-dimensional (3D) space the locomotion and angular spin of the freely moving heads of microswimmers and the beating patterns of their flagella over a sample volume more than two orders-of-magnitude larger compared to existing optical modalities. Using this platform, we quantified the 3D locomotion of 2133 bovine sperms and determined the spin axis and the angular velocity of the sperm head, providing the perspective of an observer seated at the moving and spinning sperm head. In this constantly transforming perspective, flagellum-beating patterns are decoupled from both the 3D translation and spin of the head, which provides the opportunity to truly investigate the 3D spatio-temporal kinematics of the flagellum. In addition to providing unprecedented information on the 3D locomotion of microswimmers, this computational imaging technique could also be instrumental for micro-robotics and sensing research, enabling the high-throughput quantification of the impact of various stimuli and chemicals on the 3D swimming patterns of sperms, motile bacteria and other micro-organisms, generating new insights into taxis behaviors and the underlying biophysics.

Indexed as

flagellar motionholographyon-chip microscopysperm head spinsperm tracking

Identifiers

PMID30839645
PMCPMC6107047

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.