ArticleLight, science & applications2018
Subnanometer localization accuracy in widefield optical microscopy.
Article in Light, science & applications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Traceable localization enables accurate integration of quantum emitters and photonic structures with high yield.Optica quantum · 2024Article
- Large-FOV 3D localization microscopy by spatially variant point spread function generation.Science advances · 2024Article
- Article
- Unmasking the Resolution-Throughput Tradespace of Focused-Ion-Beam Machining.Advanced functional materials · 2022Article
- Photon-free (s)CMOS camera characterization for artifact reduction in high- and super-resolution microscopy.Nature communications · 2022Article
- Sub-picoliter Traceability of Microdroplet Gravimetry and Microscopy.Analytical chemistry · 2022Article
- Improved localization precision via restricting confined biomolecule stochastic motion in single-molecule localization microscopy.Nanophotonics (Berlin, Germany) · 2022Article
- Accurate measurement of the photonic crystal slab period using diffraction in the Littrow configuration.Optics express · 2021Article
- Accurate localization microscopy by intrinsic aberration calibration.Nature communications · 2021Article
- Microscope calibration protocol for single-molecule microscopy.Optics express · 2021Article
- Quantifying accuracy and heterogeneity in single-molecule super-resolution microscopy.Nature communications · 2020Article
- Correcting Artifacts in Single Molecule Localization Microscopy Analysis Arising from Pixel Quantum Efficiency Differences in sCMOS Cameras.Scientific reports · 2019Article
- Nanomotor tracking experiments at the edge of reproducibility.Scientific reports · 2019Article
- Geometric-Phase Microscopy for Quantitative Phase Imaging of Isotropic, Birefringent and Space-Variant Polarization Samples.Scientific reports · 2019Article
- Particle Tracking of Microelectromechanical System Performance and Reliability.Journal of microelectromechanical systems : a joint IEEE and ASME publication on microstructures, microactuators, microsensors, and microsystems · 2018Article
- Subnanometer structure and function from ion beams through complex fluidics to fluorescent particles.Lab on a chip · 2017Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The common assumption that precision is the limit of accuracy in localization microscopy and the typical absence of comprehensive calibration of optical microscopes lead to a widespread issue-overconfidence in measurement results with nanoscale statistical uncertainties that can be invalid due to microscale systematic errors. In this article, we report a comprehensive solution to this underappreciated problem. We develop arrays of subresolution apertures into the first reference materials that enable localization errors approaching the atomic scale across a submillimeter field. We present novel methods for calibrating our microscope system using aperture arrays and develop aberration corrections that reach the precision limit of our reference materials. We correct and register localization data from multiple colors and test different sources of light emission with equal accuracy, indicating the general applicability of our reference materials and calibration methods. In a first application of our new measurement capability, we introduce the concept of critical-dimension localization microscopy, facilitating tests of nanofabrication processes and quality control of aperture arrays. In a second application, we apply these stable reference materials to answer open questions about the apparent instability of fluorescent nanoparticles that commonly serve as fiducial markers. Our study establishes a foundation for subnanometer localization accuracy in widefield optical microscopy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.