ArticleNucleic acids research2019
RNA interference may result in unexpected phenotypes in Caenorhabditis elegans.
Article in Nucleic acids research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 29 citations in OpenAlex.
- A methodology for RNAi gene knockdown in the marine annelid Streblospio benedicti by delivering dsRNA.Development (Cambridge, England) · 2026Article
- Quantifying Embryonic Lethality Rate in Caenorhabditis elegans.Journal of visualized experiments : JoVE · 2026Article
- Enhancing Late-Life Survival and Mobility via Mitohormesis by Reducing Mitochondrial Calcium Levels.Aging cell · 2025Article
- Gaultheria leucocarpa inhibits Aβ fibrillization and enhances mitophagy-mediated degradation of pathogenic proteins.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2025Article
- The immune response to RNA suppresses nucleic acid synthesis by limiting ribose 5-phosphate.The EMBO journal · 2024Article
- Regulatory Role ofInternational journal of molecular sciences · 2024Article
- An Intricate Network Involving the Argonaute ALG-1 Modulates Organismal Resistance to Oxidative Stress.Nature communications · 2024Article
- Diversification of small RNA pathways underlies germline RNA interference incompetence in wild Caenorhabditis elegans strains.Genetics · 2024Article
- Comparative transcriptome analysis of resistant and susceptible watermelon genotypes reveals the role of RNAi, callose, proteinase, and cell wall in squash vein yellowing virus resistance.Frontiers in plant science · 2024Article
- High-throughput transcriptome sequencing reveals the protective role of adenosine receptor-related genes in paraquat-exposedToxicology research · 2023Article
- Box C/D small nucleolar ribonucleoproteins regulate mitochondrial surveillance and innate immunity.PLoS genetics · 2022Article
- Functional Screening of Parkinson's Disease Susceptibility Genes to Identify Novel Modulators of α-Synuclein Neurotoxicity inFrontiers in aging neuroscience · 2022Article
- Recovery from heat shock requires the microRNA pathway in Caenorhabditis elegans.PLoS genetics · 2021Article
- Genes implicated inBiology letters · 2021Article
- The CONJUDOR pipeline for multiplexed knockdown of gene pairs identifies RBBP-5 as a germ cell reprogramming barrier in C. elegans.Nucleic acids research · 2021Article
- PhenoMIP: High-Throughput Phenotyping of DiverseG3 (Bethesda, Md.) · 2020Article
- Feeding exogenous dsRNA interferes with endogenous sRNA accumulation in Paramecium.DNA research : an international journal for rapid publication of reports on genes and genomes · 2020Article
- Nanoluciferase-Based Method for Detecting Gene Expression inGenetics · 2019Article
- Dietary sulfur amino acid restriction upregulates DICER to confer beneficial effects.Molecular metabolism · 2019Article
Corrections and comments
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Authors and funding
12 authors at 5 institutions in 3 countries.
Funding
Abstract
RNA interference (RNAi) is a valuable technique to determine gene function. In Caenorhabditis elegans, RNAi can be achieved by feeding worms bacteria carrying a plasmid expressing double-stranded RNA (dsRNA) targeting a gene of interest. The most commonly used plasmid vector for this purpose is L4440. However, it has been noticed that sequences within L4440 may elicit unspecific effects. Here, we provide a comprehensive characterization of these effects and their mechanisms and describe new unexpected phenotypes uncovered by the administration of unspecific exogenous dsRNA. An example involves dsRNA produced by the multiple cloning site (MCS) of L4440, which shares complementary sequences with some widely used reporter vectors and induces partial transgene silencing via the canonical and antiviral RNAi pathway. Going beyond transgene silencing, we found that the reduced embryonic viability of mir-35-41(gk262) mutants is partially reversed by exogenous dsRNA via a mechanism that involves canonical RNAi. These results indicate cross-regulation between different small RNA pathways in C. elegans to regulate embryonic viability. Recognition of the possible unspecific effects elicited by RNAi vectors is important for rigorous interpretation of results from RNAi-based experiments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.