ReviewJournal of experimental botany2019
New beginnings and new ends: methods for large-scale characterization of protein termini and their use in plant biology.
Review in Journal of experimental botany, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 47 citations in OpenAlex.
- Four guidelines to identify bona fide protease-substrate pairs.Journal of experimental botany · 2025Review
- Elucidating tissue and subcellular specificity of the entire SUMO network reveals how stress responses are fine-tuned in a eukaryote.Science advances · 2025Article
- Ubiquitin E3 ligases in the plant Arg/N-degron pathway.The Biochemical journal · 2024Review
- Charting the evolutionary path of the SUMO modification system in plants reveals molecular hardwiring of development to stress adaptation.The Plant cell · 2024Review
- Intra-chloroplast proteases: A holistic network view of chloroplast proteolysis.The Plant cell · 2024Review
- The lowdown on breakdown: Open questions in plant proteolysis.The Plant cell · 2024Review
- A bipartite chromatophore transit peptide and N-terminal protein processing in the Paulinella chromatophore.Plant physiology · 2022Article
- Protein Processing in Plant Mitochondria Compared to Yeast and Mammals.Frontiers in plant science · 2022Review
- Trichome Transcripts as Efficiency Control for Synthetic Biology and Molecular Farming.Methods in molecular biology (Clifton, N.J.) · 2022Article
- A Strong Cation Exchange Chromatography Protocol for Examining N-Terminal Proteoforms.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Sensitive Plant N-Terminome Profiling with HUNTER.Methods in molecular biology (Clifton, N.J.) · 2022Article
- A User Guide to Validation, Annotation, and Evaluation of N-Terminome Datasets with MANTI.Methods in molecular biology (Clifton, N.J.) · 2022Article
- PBC, an easy and efficient strategy for high-throughput protein C-terminome profiling.Frontiers in cell and developmental biology · 2022Article
- N-Terminomics Strategies for Protease Substrates Profiling.Molecules (Basel, Switzerland) · 2021Review
- Abundance of metalloprotease FtsH12 modulates chloroplast development in Arabidopsis thaliana.Journal of experimental botany · 2021Article
- MANTI: Automated Annotation of Protein N-Termini for Rapid Interpretation of N-Terminome Data Sets.Analytical chemistry · 2021Article
- Cellular Control of Protein Turnover via the Modification of the Amino Terminus.International journal of molecular sciences · 2021Review
- To New Beginnings: Riboproteogenomics Discovery of N-Terminal Proteoforms inFrontiers in plant science · 2021Article
- Expression of Colorectal Cancer Antigenic Protein Fused to IgM Fc in Chinese Cabbage (Plants (Basel, Switzerland) · 2020Article
- The Mouse Heart Mitochondria N Terminome Provides Insights into ClpXP-Mediated Proteolysis.Molecular & cellular proteomics : MCP · 2020Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dynamic regulation of protein function and abundance plays an important role in virtually every aspect of plant life. Diversifying mechanisms at the RNA and protein level result in many protein molecules with distinct sequence and modification, termed proteoforms, arising from a single gene. Distinct protein termini define proteoforms arising from translation of alternative transcripts, use of alternative translation initiation sites, and different co- and post-translational modifications of the protein termini. Also site-specific proteolytic processing by endo- and exoproteases generates truncated proteoforms, defined by distinct protease-generated neo-N- and neo-C-termini, that may exhibit altered activity, function, and localization compared with their precursor proteins. In eukaryotes, the N-degron pathway targets cytosolic proteins, exposing destabilizing N-terminal amino acids and/or destabilizing N-terminal modifications for proteasomal degradation. This enables rapid and selective removal not only of unfolded proteins, but also of substrate proteoforms generated by proteolytic processing or changes in N-terminal modifications. Here we summarize current protocols enabling proteome-wide analysis of protein termini, which have provided important new insights into N-terminal modifications and protein stability determinants, protein maturation pathways, and protease-substrate relationships in plants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.