ArticleScientific reports2019
Selection of suitable reference genes for gene expression analysis in gills and liver of fish under field pollution conditions.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 36 citations in OpenAlex.
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- Dietary genistein alleviates arsenic trioxide-induced ionoregulatory disruption, oxidative and endoplasmic reticulum stress, and apoptotic gene expression in the gills and kidneys of Oreochromis niloticus.Veterinary research communications · 2026Article
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- Genistein supplementation attenuates arsenic-triggered neurobehavioral deficits and ER stress-associated gene dysregulation in Nile tilapia.Fish physiology and biochemistry · 2025Article
- Comparative study of fish adaptation at cellular and genetic levels across eco-sensitive zones with contrasting oxygen and temperature regimes.Scientific reports · 2025Article
- Molecular docking prediction and hepatoprotective potential of genistein-enriched diet against arsenic toxicity in juvenile nile tilapia: implications for ER stress and oxidative damage.Veterinary research communications · 2025Article
- Evaluation of reference genes for gene expression analysis in Japanese flounder (Paralichthys olivaceus) under temperature stress.BMC genomics · 2025Article
- Physiological, biochemical, apoptosis-linked gene expression, and histopathological insights in Nile catfish subjected to toxicity by magnetite nanogel.Fish physiology and biochemistry · 2025Article
- Seasonal Stability Assessment of Reference Genes for Quantitative Real-Time Polymerase Chain Reaction Normalization inCurrent issues in molecular biology · 2024Article
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- Distress Regulates Different Pathways in the Brain of Common Carp: A Preliminary Study.Animals : an open access journal from MDPI · 2021Article
- Temporal changes in gene expression and genotype frequency of the ornithine decarboxylase gene in native silversideEvolutionary applications · 2020Article
- Selection of candidate reference genes for RT-qPCR analysis in Argulus siamensis and their validation through screening of drugs and drug targets.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To understand the role of gene expression in adaptive variation, it is necessary to examine expression variation in an ecological context. Quantitative real-time PCR (qPCR) is considered the most accurate and reliable technique to measure gene expression and to validate the data obtained by RNA-seq; however, accurate normalization is crucial. In Chile, the freshwater silverside fish Basilichthys microlepidotus inhabits both polluted and nonpolluted areas, showing differential gene expression related to pollution. In this study, we infer the stability of six potential reference genes (tubulin alpha, hypoxanthine-guanine phosphoribosyltransferase, glyceraldehyde-3-phosphate dehydrogenase, beta-actin, 60S ribosomal protein L13, and 60S ribosomal protein L8) in the gills and liver of silverside individuals inhabiting polluted and nonpolluted areas. To validate the reference genes selected, the most and least stable reference genes were used to normalize two target transcripts, one for each organ. The RefFinder tool was used to analyze and identify the most stably expressed genes. The 60S ribosomal protein L8 gene was ranked as the most stable gene for both organs. Our results show that reference gene selection influences the detection of differences in the expression levels of target genes in different organs and, also highlighting candidate reference genes that could be used in field studies.
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