ArticleScientific reports2019
Calcium Involved Directional Organization of Polymer Chains in Polyester Nanogranules in Bacterial Cells.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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1 citing paper in PubMed, 8 citations in OpenAlex.
- Analytical Techniques for Melanin Identification and Estimation by Mass Spectrometry.Methods in molecular biology (Clifton, N.J.) · 2025Article
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Authors and funding
6 authors at 2 institutions in 3 countries.
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No grant is acknowledged in the PubMed record.
Abstract
Soil bacteria accumulate polyesters (typically poly([R]-3-hydroxybutyrate (PHB), in which one end of the chain terminates with a carboxyl group) in the form of hydrated, amorphous nanogranules in cells. However, it is not clear what drives the structure of these biomaterials inside bacterial cells. Here, we determined that calcium guides intracellular formation of PHB nanogranules. Our systematic study using the surface zeta potential measurement and the carboxyl-specific SYTO-62 dye binding assay showed that the terminal carboxyl is not exposed to the granule surface but is buried inside native "unit-granules" comprising the mature granule. Extracellular Ca
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