ArticleScientific reports2019
Unexpected genomic rearrangements at targeted loci associated with CRISPR/Cas9-mediated knock-in.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 33 citations in OpenAlex.
- Genetic quality assurance and genetic monitoring of laboratory mice and rats: FELASA Working Group Report.Laboratory animals · 2020Guideline
- Genomically integrated orthogonal translation system in Escherichia coli enables production of functional modified [NiFe]-hydrogenases.Microbial cell factories · 2026Article
- Megabase-scale loss of heterozygosity provoked by CRISPR-Cas9 DNA double-strand breaks.Molecular cell · 2025Article
- Next-generation CRISPR technology for genome, epigenome and mitochondrial editing.Transgenic research · 2024Review
- Decoding the complexity of on-target integration: characterizing DNA insertions at the CRISPR-Cas9 targeted locus using nanopore sequencing.BMC genomics · 2024Article
- Cell cycle arrest and p53 prevent ON-target megabase-scale rearrangements induced by CRISPR-Cas9.Nature communications · 2023Article
- Article
- CRISPR/Cas9 unveils the dynamics of the endogenous µ-opioid receptors on neuronal cells under continuous opioid stimulation.Pharmacology research & perspectives · 2022Article
- Evaluation of twoMolecular therapy. Methods & clinical development · 2021Article
- Single-strand template repair: key insights to increase the efficiency of gene editing.Current genetics · 2021Review
- A reporter system for enriching CRISPR/Cas9 knockout cells in technically challenging settings like patient models.Scientific reports · 2021Article
- CRISPR, animals, and FDA oversight: Building a path to success.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Assessment of Mosaicism and Detection of Cryptic Alleles in CRISPR/Cas9-Engineered Neurofibromatosis Type 1 andFrontiers in genetics · 2021Article
- Anticipating and Identifying Collateral Damage in Genome Editing.Trends in genetics : TIG · 2020Review
- A most formidable arsenal: genetic technologies for building a better mouse.Genes & development · 2020Review
- CRISPR Start-Loss: A Novel and Practical Alternative for Gene Silencing through Base-Editing-Induced Start Codon Mutations.Molecular therapy. Nucleic acids · 2020Article
- CRISPR-based strategies for targeted transgene knock-in and gene correction.Faculty reviews · 2020Review
- Article
- Variation in zygotic CRISPR/Cas9 gene editing outcomes generates novel reporter and deletion alleles at the Gdf11 locus.Scientific reports · 2019Article
- Gene editing: Towards the third generation of adoptive T-cell transfer therapies.Immuno-oncology technology · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The CRISPR/Cas9 gene editing tool enables accessible and efficient modifications which (re)ignited molecular research in certain species. However, targeted integration of large DNA fragments using CRISPR/Cas9 can still be challenging in numerous models. To systematically compare CRISPR/Cas9's efficiency to classical homologous recombination (cHR) for insertion of large DNA fragments, we thoroughly performed and analyzed 221 experiments targeting 128 loci in mouse ES cells. Although both technologies proved efficient, CRISPR/Cas9 yielded significantly more positive clones as detected by overlapping PCRs. It also induced unexpected rearrangements around the targeted site, ultimately rendering CRISPR/Cas9 less efficient than cHR for the production of fully validated clones. These data show that CRISPR/Cas9-mediated recombination can induce complex long-range modifications at targeted loci, thus emphasizing the need for thorough characterization of any genetically modified material obtained through CRISPR-mediated gene editing before further functional studies or therapeutic use.
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Registered trials
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