ArticleScientific reports2019
CRISPR/CAS9 targeted CAPTURE of mammalian genomic regions for characterization by NGS.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- Prospects of multipurpose biomonitoring for fisheries assessment based on environmental nucleic acids.Journal of fish biology · 2026Review
- From Panels to Pathogen Networks: The Expanding Role of Targeted Sequencing in Veterinary Medicine.Biology · 2025Review
- Techniques to detect physical interactions between genomic regions.Epigenomics · 2025Review
- Article
- Development and Characterization of the NISTCHO Reference Cell Line.Biotechnology journal · 2025Article
- Next-generation sequencing: A powerful multi-purpose tool in cell line development for biologics production.Computational and structural biotechnology journal · 2025Review
- Not Only Editing: A Cas-Cade of CRISPR/Cas-Based Tools for Functional Genomics in Plants and Animals.International journal of molecular sciences · 2024Review
- Quantitative imaging of chromatin inheritance using a dual-color histone inSTAR protocols · 2022Article
- Cas12a-Capture: A Novel, Low-Cost, and Scalable Method for Targeted Sequencing.The CRISPR journal · 2022Article
- Enhancing stability of recombinant CHO cells by CRISPR/Cas9-mediated site-specific integration into regions with distinct histone modifications.Frontiers in bioengineering and biotechnology · 2022Article
- Review
- RGEN-seq for highly sensitive amplification-free screen of off-target sites of gene editors.Scientific reports · 2021Article
- Detection of genetic variation and base modifications at base-pair resolution on both DNA and RNA.Communications biology · 2021Article
- CaBagE: A Cas9-based Background Elimination strategy for targeted, long-read DNA sequencing.PloS one · 2021Article
- Versatile 3' Functionalization of CRISPR Single Guide RNA.Chembiochem : a European journal of chemical biology · 2020Article
- Effect of Exogenous Transcription Factors Integration Sites on Safety and Pluripotency of Induced Pluripotent Stem Cells.Balkan journal of medical genetics : BJMG · 2020Article
- Microfluidic automated plasmid library enrichment for biosynthetic gene cluster discovery.Nucleic acids research · 2020Article
- Novel CRISPR-based sequence specific enrichment methods for target loci and single base mutations.PloS one · 2020Article
- Purification of specific DNA species using the CRISPR system.Biology methods & protocols · 2019Review
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The robust detection of structural variants in mammalian genomes remains a challenge. It is particularly difficult in the case of genetically unstable Chinese hamster ovary (CHO) cell lines with only draft genome assemblies available. We explore the potential of the CRISPR/Cas9 system for the targeted capture of genomic loci containing integrated vectors in CHO-K1-based cell lines followed by next generation sequencing (NGS), and compare it to popular target-enrichment sequencing methods and to whole genome sequencing (WGS). Three different CRISPR/Cas9-based techniques were evaluated; all of them allow for amplification-free enrichment of target genomic regions in the range from 5 to 60 fold, and for recovery of ~15 kb-long sequences with no sequencing artifacts introduced. The utility of these protocols has been proven by the identification of transgene integration sites and flanking sequences in three CHO cell lines. The long enriched fragments helped to identify Escherichia coli genome sequences co-integrated with vectors, and were further characterized by Whole Genome Sequencing (WGS). Other advantages of CRISPR/Cas9-based methods are the ease of bioinformatics analysis, potential for multiplexing, and the production of long target templates for real-time sequencing.
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