ArticleBMC infectious diseases2019
A rapid and sensitive recombinase aided amplification assay to detect hepatitis B virus without DNA extraction.
Article in BMC infectious diseases, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 101 citations in OpenAlex.
- Rapid Detection ofMicroorganisms · 2026Article
- Establishment and Application of Duplex Recombinase-Aided Amplification Combined with Lateral Flow Dipsticks for Rapid and Simultaneous Visual Detection ofFoods (Basel, Switzerland) · 2025Article
- Progress in the application of isothermal amplification technology in the diagnosis of infectious diseases.Frontiers in microbiology · 2025Review
- A comparative study of extraction free detection of HBV DNA using sodium dodecyl sulfate, N-lauroylsarcosine sodium salt, and sodium dodecyl benzene sulfonate.Scientific reports · 2024Article
- Article
- Recombinase-aided amplification coupled with lateral flow dipstick for efficient and accurate detection of Bombyx mori nucleopolyhedrovirus.Folia microbiologica · 2024Article
- Detection method for reverse transcription recombinase-aided amplification of avian influenza virus subtypes H5, H7, and H9.BMC veterinary research · 2024Article
- Development of Rapid Isothermal Detection Methods Real-Time Fluorescence and Lateral Flow Reverse Transcription Recombinase-Aided Amplification Assay for Bovine Coronavirus.Transboundary and emerging diseases · 2024Article
- Point-of-Care Testing for Hepatitis Viruses: A Growing Need.Life (Basel, Switzerland) · 2023Review
- Development of a recombinase-aided amplification method combined with lateral flow dipstick assay to detectVeterinary world · 2023Article
- Real-time recombinase-aided amplification assay for rapid amplification of the IS1081 gene of Mycobacterium tuberculosis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2023Article
- A one-step reverse-transcription recombinase aided PCR assay for the rapid and sensitive detection of human enteroviruses.Biosafety and health · 2023Article
- Rapid two-stage amplification in a single tube for simultaneous detection of norovirus GII and group a rotavirus.Journal of clinical laboratory analysis · 2023Article
- Rapid Onsite Visual Detection of Orf Virus Using a Recombinase-Aided Amplification Assay.Life (Basel, Switzerland) · 2023Article
- HIV infection detection using CRISPR/Cas systems: Present and future prospects.Computational and structural biotechnology journal · 2023Review
- Advances in the application of recombinase-aided amplification combined with CRISPR-Cas technology in quick detection of pathogenic microbes.Frontiers in bioengineering and biotechnology · 2023Review
- Real-Time Reverse Transcription Recombinase-Aided Amplification Assay for Rapid Amplification of theInternational journal of molecular sciences · 2022Article
- Article
- Advances in Biosensing Technologies for Diagnosis of COVID-19.Biosensors · 2022Review
- Manually-Operated, Slider Cassette for Multiplexed Molecular Detection at the Point of Care.Sensors and actuators. B, Chemical · 2022Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundHepatitis B virus (HBV) infection is the major public health problem worldwide. In clinical practice, serological and molecular assays are the most commonly used diagnostic methods to detect HBV infection in clinical practices.
methodsHere we present a rapid and sensitive recombinase aided amplification assay (RAA) to detect HBV at 39.0 °C for 30 min without DNA extraction from serum samples. The analytical sensitivity of RAA assay was 100 copies per reaction and showed no cross reaction with human immunodeficiency virus (HIV) and hepatitis C virus (HCV). The universality of RAA assay was validated by testing of 41 archived serum samples with predefined HBV genotypes (B, C and D).
resultsA total of 130 archived suspected HBV infected serum samples were detected by commercial qPCR with DNA extraction and RAA assay without DNA extraction (heat-treatment). Compared with qPCR assay as a reference, the RAA assay obtained 95.7% sensitivity and 100% specificity and a kappa value of 0.818.
conclusionsWe developed a rapid, convenient, highly sensitive and specific method to detect HBV without DNA extraction in clinical samples. This RAA method of HBV detection is very suitable for clinical testing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.