ArticleRNA biology2019
Alternative splicing regulation of doublesex gene by RNA-binding proteins in the silkworm Bombyx mori.
Article in RNA biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Molecular mechanisms of sex determination in Lepidoptera: current status and perspectives.Insect science · 2026Review
- Non-parasite genome encoded virus-like RNAs reprogram the pathogenicity of human blood flukes.Nature communications · 2025Article
- Understanding the genetics of sex determination in insects and its relevance to genetic pest management.Insect molecular biology · 2025Review
- Schistosoma sex-biased microRNAs regulate ovarian development and egg production by targeting Wnt signaling pathway.Communications biology · 2024Article
- Bmlark is essential for embryonic development.BMC genomics · 2024Article
- Alternative splicing as a source of phenotypic diversity.Nature reviews. Genetics · 2022Review
- CHARGE syndrome-associated proteins FAM172A and CHD7 influence male sex determination and differentiation through transcriptional and alternative splicing mechanisms.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- CELF1 represses Doublesex1 expression via its 5' UTR in the crustacean Daphnia magna.PloS one · 2022Article
- Novel Doublesex Duplication Associated with Sexually Dimorphic Development of Dogface Butterfly Wings.Molecular biology and evolution · 2021Article
- Evolution of sexual development and sexual dimorphism in insects.Current opinion in genetics & development · 2021Review
- Long Non-Coding RNAs in Insects.Animals : an open access journal from MDPI · 2021Review
- femaleless Controls Sex Determination and Dosage Compensation Pathways in Females of Anopheles Mosquitoes.Current biology : CB · 2021Article
- The Sex Determination Cascade in the Silkworm.Genes · 2021Review
- Transgenic and knockout analyses of Masculinizer and doublesex illuminated the unique functions of doublesex in germ cell sexual development of the silkworm, Bombyx mori.BMC developmental biology · 2020Article
- Spliceosomal Protein GeneInternational journal of molecular sciences · 2020Article
- Masc-induced dosage compensation in silkworm cultured cells.FEBS open bio · 2019Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Doublesex is highly conserved and sex-specifically spliced in insect sex-determination pathways, and its alternative splicing (AS) is regulated by Transformer, an exonic splicing activator, in the model system of Drosophila melanogaster. However, due to the lack of a transformer gene, AS regulation of doublesex remains unclear in Lepidoptera, which contain the economically important silkworm Bombyx mori and thousands of agricultural pests. Here, we use yeast three-hybrid system to screen for RNA-binding proteins that recognize sex-specific exons 3 and 4 of silkworm doublesex (Bm-dsx); this approach identified BxRBP1/Lark binding to the exon 3, and BxRBP2/TBPH and BxRBP3/Aret binding to the exon 4. Investigation of tissues shows that BxRBP1 and BxRBP2 have no sex specificity, but BxRBP3 has - three of its four isoforms are expressed with a sex-bias. Using novel sex-specific silkworm cell lines, we find that BxRBP1 and BxRBP3 directly interact with each other, and cooperatively function as splicing repressors. Over-expression of BxRBP1 and BxRBP3 isoforms efficiently inhibits splicing of the exons 3 and 4 in the female-specific cells and generates the male-specific isoform of Bm-dsx. We also demonstrate that the sex-determination upstream gene Masc regulates alternatively transcribed BxRBP3 isoforms. Thus, we identify a new regulatory mechanism of doublesex AS in the silkworm, revealing an evolutionary divergence in insect sex-determination.
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