Evidence map›Paper›PMID 30836724›Full record

ArticleInternational journal of stem cells2019

Effect of Substrate Topography and Chemistry on Human Mesenchymal Stem Cell Markers: A Transcriptome Study.

Bo Zhang, Naresh Kasoju, Qiongfang Li, Jinmin Ma, Aidong Yang, Zhanfeng Cui, Hui Wang, Hua Ye

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of stem cells, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
2.3field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 21 citations in OpenAlex.

  1. Article
  2. The clinical potential of meniscal progenitor cells.The journal of cartilage & joint preservation · 2024
    Review
  3. Article
  4. Review
  5. Gene: X · 2020
    Article
  6. Dynamics of trophoblast differentiation in peri-implantation-stage human embryos.Proceedings of the National Academy of Sciences of the United States of America · 2019
    Article
  7. Review
  8. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Bo ZhangInstitute of Biomedical Engineering, Department of Engineering Science, University of Oxford, Oxford, UK.
Naresh KasojuInstitute of Biomedical Engineering, Department of Engineering Science, University of Oxford, Oxford, UK.
Qiongfang LiBGI-Shenzhen, Shenzhen 518083, China.
Jinmin MaBGI-Shenzhen, Shenzhen 518083, China.
Aidong YangDepartment of Engineering Science, University of Oxford, Oxford, UK.
Zhanfeng CuiInstitute of Biomedical Engineering, Department of Engineering Science, University of Oxford, Oxford, UK.
Hui WangInstitute of Biomedical Engineering, Department of Engineering Science, University of Oxford, Oxford, UK.
Hua YeInstitute of Biomedical Engineering, Department of Engineering Science, University of Oxford, Oxford, UK.
University of Oxford · GBBGI Group (China) · CN

Funding

Biotechnology and Biological Sciences Research Council BB/H008608/1
6 · The paper itself

Abstract

BACKGROUND AND

objectivesThe International Society for Cellular Therapy (ISCT) proposed a set of minimal markers for identifying human mesenchymal stromal cells (hMSCs) in 2007. Since then, with the growing interest of better characterising hMSCs, various additional surface markers have been proposed. However, the impact of how culture conditions, in particular, the culture surface, vary the expression of hMSC markers was overlooked. METHODS AND

resultsIn this study, we utilized the RNA sequencing data on hMSCs cultured on different surfaces to investigate the variation of the proposed hMSC biomarkers. One of the three ISCT proposed positive biomarker, CD90 was found to be significantly down regulated on hMSCs culture on fibrous surfaces when compared to flat surfaces. The detected gene expression values for 177 hMSCs biomarkers compiled from the literature are reported here. Correlation and cluster analysis revealed the existence of different biomarker communities that displayed a similar expression profile. We found a list of hMSCs biomarkers which are the least sensitive to a change in surface properties and another list of biomarkers which are found to have high sensitivity to a change in surface properties.

conclusionsThis study demonstrated that substrate properties have paramount effect on altering the expressions of hMSCs biomarkers and the proposed list of substrate-stable and substrate-sensitive biomarkers would better assist in the population characterisation. However, proteomic level analysis would be essential to confirm the observations noted.

Indexed as

Cell biomaterial interactionsHuman mesenchymal stromal cellsNext generation sequencingQuality controlRegenerative medicineSurface markers

Identifiers

PMID30836724
PMCPMC6457710
OpenAlexW2915668333

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.