Evidence map›Paper›PMID 30836606›Full record

ArticleInternational journal of molecular sciences2019

Vascularized Bone-Mimetic Hydrogel Constructs by 3D Bioprinting to Promote Osteogenesis and Angiogenesis.

Takahisa Anada, Chi-Chun Pan, Alexander M Stahl, Satomi Mori, Junji Fukuda, Osamu Suzuki, Yunzhi Yang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.

0numbers the graph read from it
0cells of the map it votes in
78citing papers in PubMed
7.4field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

78 citing papers in PubMed, 153 citations in OpenAlex.

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  11. Guidance on biomaterials for periodontal tissue regeneration: Fabrication methods, materials and biological considerations.Dental materials : official publication of the Academy of Dental Materials · 2025
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18 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 2 countries.

Takahisa AnadaSoft Materials Chemistry, Department of Applied Chemistry, Institute for Materials Chemistry and Engineering, Graduate School of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan. takahisa_anada@ms.ifoc.kyushu-u.ac.jp.
Chi-Chun PanDepartment of Orthopaedic Surgery, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, CA 94305, USA. chichun@stanford.edu.
Alexander M StahlDepartment of Orthopaedic Surgery, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, CA 94305, USA. amstahl@stanford.edu.
Satomi MoriGraduate School of Dentistry, Tohoku University, 4-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan. satomi.mori.p7@dc.tohoku.ac.jp.
Junji FukudaFaculty of Engineering, Yokohama National University, 79-5 Tokiwadai, Hodogaya-ku, Yokohama 240-8501, Japan. fukuda@ynu.ac.jp.
Osamu SuzukiDivision of Craniofacial Function Engineering, Tohoku University Graduate School of Dentistry, 4-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan. suzuki-o@m.tohoku.ac.jp.
Yunzhi YangDepartment of Orthopaedic Surgery, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, CA 94305, USA. ypyang@stanford.edu.ORCID 0000-0002-3891-0574
Stanford University · USTohoku University · JPKyushu University · JPYokohama National University · JP

Funding

Grants-in Aid from the Ministry of Education, Science, Sports, and Culture of Japan 17H02095
6 · The paper itself

Abstract

Bone is a highly vascularized tissue with a unique and complex structure. Long bone consists of a peripheral cortical shell containing a network of channels for vascular penetration and an inner highly vascularized bone marrow space. Bioprinting is a powerful tool to enable rapid and precise spatial patterning of cells and biomaterials. Here we developed a two-step digital light processing technique to fabricate a bone-mimetic 3D hydrogel construct based on octacalcium phosphate (OCP), spheroids of human umbilical vein endothelial cells (HUVEC), and gelatin methacrylate (GelMA) hydrogels. The bone-mimetic 3D hydrogel construct was designed to consist of a peripheral OCP-containing GelMA ring to mimic the cortical shell, and a central GelMA ring containing HUVEC spheroids to mimic the bone marrow space. We further demonstrate that OCP, which is evenly embedded in the GelMA, stimulates the osteoblastic differentiation of mesenchymal stem cells. We refined the design of a spheroid culture device to facilitate the rapid formation of a large number of HUVEC spheroids, which were embedded into different concentrations of GelMA hydrogels. It is shown that the concentration of GelMA modulates the extent of formation of the capillary-like structures originating from the HUVEC spheroids. This cell-loaded hydrogel-based bone construct with a biomimetic dual ring structure can be potentially used for bone tissue engineering.

Indexed as

BiomimeticsBioprintingBlood VesselsBone and BonesBone DevelopmentGelatinHumansHuman Umbilical Vein Endothelial CellsHydrogelsNeovascularization, PhysiologicOsteogenesisPolyhydroxyethyl MethacrylatePrinting, Three-DimensionalGelatinHydrogelsPolyhydroxyethyl Methacrylate3D bioprintinggelatin methacrylate (GelMA)octacalcium phosphate (OCP)spheroids

Identifiers

PMID30836606
PMCPMC6429349
OpenAlexW2919078487

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.