ArticleJournal of leukocyte biology2019
Real-time deformability cytometry reveals sequential contraction and expansion during neutrophil priming.
Article in Journal of leukocyte biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 61 citations in OpenAlex.
- High-throughput mechanomic screening reveals novel regulators of single-cell mechanics.Biophysical journal · 2026Article
- Synthetic Cell-Based Artificial Stem Cell Niches for Hematopoietic Stem Cell Differentiation.Chembiochem : a European journal of chemical biology · 2026Article
- Review
- Integrating single-cell biophysical and transcriptomic features to resolve functional heterogeneity in mantle cell lymphoma.Science advances · 2025Article
- Whole blood biophysical immune profiling of newborn infants correlates with immune responses.Pediatric research · 2025Article
- Rapid autofluorescence flow cytometric analysis of agonist-induced neutrophil and eosinophil polarization reveals novel insights into 5-oxo-ETE-mediated granulocyte activation.Journal of inflammation (London, England) · 2025Article
- Longitudinal associations between depressive symptoms and cell deformability: do glucocorticoids play a role?European archives of psychiatry and clinical neuroscience · 2025Article
- Cell trajectory modulation: rapid microfluidic biophysical profiling of CAR T cell functional phenotypes.Nature communications · 2025Article
- Outer membrane vesicles derived from probioticFrontiers in immunology · 2025Article
- Chromatin changes associated with neutrophil extracellular trap formation in whole blood reflect complex immune signaling.Frontiers in immunology · 2025Article
- Cannabidiol polarizes human neutrophils toward a cancer-promoting phenotype.Frontiers in immunology · 2025Article
- Elasticity of Carrier Fluid: A Key Factor Affecting Mechanical Phenotyping in Deformability Cytometry.Micromachines · 2024Article
- Multiplexed Single-Cell Rheology Probing Using Surface Acoustic Waves.Small science · 2024Article
- Nanoparticles containing intracellular proteins modulate neutrophil functional and phenotypic heterogeneity.Frontiers in immunology · 2024Article
- M-CSF directs myeloid and NK cell differentiation to protect from CMV after hematopoietic cell transplantation.EMBO molecular medicine · 2023Article
- Orbital inflammatory pseudotumor: new advances in diagnosis, pathogenesis, and treatment.European journal of medical research · 2023Review
- Article
- Measuring cell deformation by microfluidics.Frontiers in bioengineering and biotechnology · 2023Review
- Microparticles with tunable, cell-like properties for quantitative acoustic mechanophenotyping.Microsystems & nanoengineering · 2023Article
- Molecular determinants of intrinsic cellular stiffness in health and disease.Biophysical reviews · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 3 institutions in 3 countries.
Funding
Abstract
It has become increasingly apparent that the biomechanical properties of neutrophils impact on their trafficking through the circulation and in particularly through the pulmonary capillary bed. The retention of polarized or shape-changed neutrophils in the lungs was recently proposed to contribute to acute respiratory distress syndrome pathogenesis. Accordingly, this study tested the hypothesis that neutrophil priming is coupled to morpho-rheological (MORE) changes capable of altering cell function. We employ real-time deformability cytometry (RT-DC), a recently developed, rapid, and sensitive way to assess the distribution of size, shape, and deformability of thousands of cells within seconds. During RT-DC analysis, neutrophils can be easily identified within anticoagulated "whole blood" due to their unique granularity and size, thus avoiding the need for further isolation techniques, which affect biomechanical cell properties. Hence, RT-DC is uniquely suited to describe the kinetics of MORE cell changes. We reveal that, following activation or priming, neutrophils undergo a short period of cell shrinking and stiffening, followed by a phase of cell expansion and softening. In some contexts, neutrophils ultimately recover their un-primed mechanical phenotype. The mechanism(s) underlying changes in human neutrophil size are shown to be Na
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.