Evidence map›Paper›PMID 30834995›Full record

ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2019

Role of Mycobacterium avium lysate INF-γ, IL-17, and IL-2 ELISPOT assays in diagnosing nontuberculous mycobacteria lymphadenitis in children.

Chiara Della Bella, Elisabetta Venturini, Savannah Devente, Paola Piccini, Simona Tapinassi, Leila Bianchi, Alessia Grassi, Marisa Benagiano, Heba F Mustafa Alnwaisri, Carlotta Montagnani and 5 more

Open access · bronzeAbstract readComparative Study
PubMed Publisher
In one paragraph

Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. PD-1 Inhibitor for DisseminatedOpen forum infectious diseases · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 3 institutions in 2 countries.

Chiara Della BellaDepartment of Experimental and Clinical Medicine, University of Florence, 50139, Florence, Italy.
Elisabetta VenturiniDepartment of Health Sciences, University of Florence, Anna Meyer Children's University Hospital, viale Pieraccini 24, I-50139, Florence, Italy.
Savannah DeventeVrije University, 1081 HV, Amsterdam, The Netherlands.
Paola PicciniDepartment of Health Sciences, University of Florence, Anna Meyer Children's University Hospital, viale Pieraccini 24, I-50139, Florence, Italy.
Simona TapinassiDepartment of Experimental and Clinical Medicine, University of Florence, 50139, Florence, Italy.
Leila BianchiDepartment of Health Sciences, University of Florence, Anna Meyer Children's University Hospital, viale Pieraccini 24, I-50139, Florence, Italy.
Alessia GrassiDepartment of Experimental and Clinical Medicine, University of Florence, 50139, Florence, Italy.
Marisa BenagianoDepartment of Experimental and Clinical Medicine, University of Florence, 50139, Florence, Italy.
Heba F Mustafa AlnwaisriDepartment of Experimental and Clinical Medicine, University of Florence, 50139, Florence, Italy.
Carlotta MontagnaniDepartment of Health Sciences, University of Florence, Anna Meyer Children's University Hospital, viale Pieraccini 24, I-50139, Florence, Italy.
Elena ChiappiniDepartment of Health Sciences, University of Florence, Anna Meyer Children's University Hospital, viale Pieraccini 24, I-50139, Florence, Italy.
Wilbert BitterVrije University, 1081 HV, Amsterdam, The Netherlands.
Mario M D'EliosDepartment of Experimental and Clinical Medicine, University of Florence, 50139, Florence, Italy.
Maurizio de MartinoDepartment of Health Sciences, University of Florence, Anna Meyer Children's University Hospital, viale Pieraccini 24, I-50139, Florence, Italy.
Luisa GalliDepartment of Health Sciences, University of Florence, Anna Meyer Children's University Hospital, viale Pieraccini 24, I-50139, Florence, Italy. luisa.galli@unifi.it.
University of Florence · ITMeyer Children's Hospital · ITVrije Universiteit Amsterdam · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Nontuberculous mycobacteria are the most frequent cause of chronic cervical lymphadenitis in childhood. The aim of the study was to evaluate the performance of IL-2, IL-17, and INF-γ in-house enzyme-linked immunospot assays using a Mycobacterium avium lysate, in order to identify a noninvasive diagnostic method of nontuberculous mycobacteria infection. Children with subacute and chronic lymphadenopathies or with a previous diagnosis of nontuberculous mycobacteria lymphadenitis were prospectively enrolled in the study. Sixty children with lymphadenitis were included in our study: 16 with confirmed infection (group 1), 30 probable infected (group 2) and 14 uninfected (group 3). Significantly higher median cytokine values were found in group 1 vs group 2, in group 1 vs group 3, and in group 2 vs group 3 considering IL-2-based enzyme-linked immunospot assay (p = 0.015, p < 0.001, p = 0.004, respectively). INF-γ-based enzyme-linked immunospot assay results were significantly higher in group 2 vs group 3 (p = 0.010). Differences between infected and uninfected children were not significant considering IL-17 assays (p = 0.431). Mycobacterium avium lysate IL-2 and INF-γ-based enzyme-linked immunospot assays seem to be promising noninvasive diagnostic techniques for discriminating children with nontuberculous mycobacteria lymphadenitis and noninfected subjects.

Indexed as

AdolescentBiomarkersChildChild, PreschoolCytokinesDiagnostic Tests, RoutineEnzyme-Linked Immunospot AssayFemaleHumansInfantInfant, NewbornInterferon-gammaInterleukin-17Interleukin-2LymphadenitisMaleBiomarkersCytokinesInterferon-gammaInterleukin-17Interleukin-2ChildrenELISPOTLymphadenitisMycobacterium aviumNontuberculous mycobacteria

Identifiers

PMID30834995
OpenAlexW2918590282

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.